Rabbit TRIM72 Polyclonal Antibody | anti-TRIM72 antibody

TRIM72, CT (TRIM72, MG53, Tripartite motif-containing protein 72, Mitsugumin-53) (PE)

Cat. Num. AAA6358604

• Gene Names: TRIM72; MG53
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TRIM72; Polyclonal Antibody; CT (TRIM72; MG53; Tripartite motif-containing protein 72; Mitsugumin-53) (PE); Mitsugumin-53; anti-TRIM72 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIM72. Species Crossreactivity: mouse, rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TRIM72 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 299-327 in the C-terminal region of human TRIM72, UniProt Accession #Q6ZMU5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292 cell line lysates (35ug/lane) usingMBS6008187. This demonstrates that MBS6008187 detected the TRIM72 protein (arrow).)

Testing Data

(All lanes: MBS6008187 (1:2000) Lane 1: HCT116 whole cell lysate Lane 2: human skeletal muscle lysate Lane 3: mouse skeletal muscle lysate Lane 4: rat skeletal muscle lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 53kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6008187 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008187 for immunohistochemistry.)

Product Categories/Family for anti-TRIM72 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

1. Park, E.Y., et al. Proteins 78(3):790-795(2010). 2. Han, S., et al. Hum. Mol. Genet. 18(6):1171-1180(2009). 3. Martin, J., et al. Nature 432(7020):988-994(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 270265876
• NCBI GeneID: 493829
• NCBI Accession #: NP_001008275.2
• NCBI GenBank Nucleotide #: NM_001008274.3
• UniProt Accession #: Q6ZMU5; Q8N4X6; Q8NBD9
• Molecular Weight: 53
• NCBI Official Full Name: tripartite motif-containing protein 72
• NCBI Official Synonym Full Names: tripartite motif containing 72
• NCBI Official Symbol: TRIM72
• NCBI Official Synonym Symbols: MG53
• NCBI Protein Information: tripartite motif-containing protein 72
• UniProt Protein Name: Tripartite motif-containing protein 72
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM72
• UniProt Synonym Gene Names: Mg53
• UniProt Entry Name: TRI72_HUMAN