Rabbit anti-Human SIPA1 Polyclonal Antibody | anti-SIPA1 antibody

SIPA1 Antibody

Cat. Num. AAA7105105

• Gene Names: SIPA1; SPA1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: SIPA1; Polyclonal Antibody; SIPA1 Antibody; Signal-induced proliferation-associated protein 1; Sipa-1; GTPase-activating protein Spa-1; p130 SPA-1; SPA1; anti-SIPA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1042

• Applicable Applications for anti-SIPA1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Signal-induced proliferation-associated protein 1 protein (759-927AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HL-60 whole cell lysateAll lanes: SIPA1 antibody at 2.7ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 112, 149 KDaObserved band size: 130 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7105105 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lymph node tissue using MBS7105105 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7105105 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-SIPA1 antibody

GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.

Product Categories/Family for anti-SIPA1 antibody

Epigenetics and Nuclear Signaling; Cell biology; Developmental biology; Signal transduction; Stem cells

NCBI and Uniprot Product Information

• NCBI GI #: 206725523
• NCBI GeneID: 6494
• NCBI Accession #: NP_006738.3
• NCBI GenBank Nucleotide #: NM_006747.3
• UniProt Accession #: Q96FS4; O14518; O60484; O60618; Q2YD83
• Molecular Weight: 112,149 Da
• NCBI Official Full Name: signal-induced proliferation-associated protein 1
• NCBI Official Synonym Full Names: signal-induced proliferation-associated 1
• NCBI Official Symbol: SIPA1
• NCBI Official Synonym Symbols: SPA1
• NCBI Protein Information: signal-induced proliferation-associated protein 1
• UniProt Protein Name: Signal-induced proliferation-associated protein 1
• Protein Family: Signal-induced proliferation-associated protein
• UniProt Gene Name: SIPA1
• UniProt Synonym Gene Names: SPA1; Sipa-1

NCBI Description

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting them to the putatively inactive GDP-bound state (PubMed:9346962). Affects cell cycle progression ().