Goat anti-Human Centaurin beta 2 Polyclonal Antibody | anti-ACAP2 antibody

Centaurin beta 2, CT (ACAP2, Ankyrin Repeat, ARFGAP with Coiled-coil ANK Repeat and PH Domains 2, CENTB2, Cnt b2, KIAA0041, Pleckstrin Homology Domains 2)

Cat. Num. AAA642412

• Gene Names: ACAP2; CENTB2; CNT-B2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Centaurin beta 2; Polyclonal Antibody; CT (ACAP2; Ankyrin Repeat; ARFGAP with Coiled-coil ANK Repeat and PH Domains 2; CENTB2; Cnt b2; KIAA0041; Pleckstrin Homology Domains 2); Anti -Centaurin beta 2; anti-ACAP2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CENTB2. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ACAP2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Western Blot: 1:32,000 & 1-3ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-EKLNRFQQDSQKF from human CENTB2 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Lung (formalin-fixed, paraffin-embedded) stained with CENTB2 antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Chemiluminescence

(Antibody (1ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ACAP2 antibody

Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.

Product Categories/Family for anti-ACAP2 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119598433
• NCBI GeneID: 23527
• UniProt Accession #: Q15057; Q8N5Z8; Q9UQR3; A8K2V4
• Molecular Weight: 88,029 Da
• NCBI Official Full Name: centaurin, beta 2, isoform CRA_b
• NCBI Official Synonym Full Names: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
• NCBI Official Symbol: ACAP2
• NCBI Official Synonym Symbols: CENTB2; CNT-B2
• NCBI Protein Information: arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2; centaurin-beta-2; centaurin, beta 2; Arf GAP with coiled coil, ANK repeat and PH domains 2
• UniProt Protein Name: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
• Protein Family: Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein
• UniProt Gene Name: ACAP2
• UniProt Synonym Gene Names: CENTB2; KIAA0041; Cnt-b2
• UniProt Entry Name: ACAP2_HUMAN

Uniprot Description

CENTB2: a member of the centaurin family of ADP-ribosylation factor directed GTPase-activating proteins. Regulates cytoskeletal and membrane trafficking events in the cell periphery. Binding of phosphoinositides to a pleckstrin homology domain may regulate subcellular localization and activity.

Protein type: Motility/polarity/chemotaxis; GAPs, ARF; GAPs

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: membrane; endosome membrane

Molecular Function: zinc ion binding; Rab GTPase binding

Biological Process: positive regulation of GTPase activity