Goat TRIM69 / Trif Polyclonal Antibody | anti-TRIM69 antibody

Goat Polyclonal to Human TRIM69 / Trif

Cat. Num. AAA244043

• Gene Names: TRIM69; Trif; HSD34; RNF36; HSD-34
• Reactivity: Bovine, Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Gibbon, Mouse, Rat, Dog, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRIM69 / Trif; Polyclonal Antibody; Goat Polyclonal to Human TRIM69 / Trif; Anti-TRIM69 / Trif Antibody (C-Terminus) IHC-plus; TRIM69; HSD34; TRIF; TRIMLESS; Tripartite motif containing 69; RING finger protein 36; Tripartite motif protein 69; Tripartite motif-containing 69; RNF36; Human TRIM69; Trif; anti-TRIM69 antibody

• Host: Goat
• Reactivity: Bovine, Gorilla, Human, Monkey, Rabbit; Predicted Reactivity: Gibbon, Mouse, Rat, Dog, Hamster, Horse, Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TRIM69 / RNF36. This antibody is expected to recognize both reported isoforms (NP_892030.3; NP_542783.2).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 500

• Applicable Applications for anti-TRIM69 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (2.5 - 3.75 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-GENKEPLHILHPQ from the C-terminus of human TRIM69 / RNF36 (NP_892030.3; NP_542783.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Bovine, Rabbit (100%); Gibbon, Mouse, Rat, Hamster, Dog, Horse, Pig (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: TRIM69 / Trif antibody was raised against synthetic peptide C-GENKEPLHILHPQ from the C-terminus of human TRIM69 / RNF36 (NP_892030.3; NP_542783.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Bovine, Rabbit (100%); Gibbon, Mouse, Rat, Hamster, Dog, Horse, Pig (92%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TRIM69 / RNF36 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TRIM69 / RNF36 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Related Product Information for anti-TRIM69 antibody

This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Product Categories/Family for anti-TRIM69 antibody

Family: Tripartite Motif

NCBI and Uniprot Product Information

• NCBI GI #: 88999601
• NCBI GeneID: 140691
• NCBI Accession #: NP_892030.3
• NCBI GenBank Nucleotide #: NM_182985.4
• UniProt Accession #: Q86WT6; Q309B0; Q4G1A5; Q6W897; Q8IYY3; Q8WY16; Q8WY17; A8MX03
• Molecular Weight: 32,068 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM69 isoform a
• NCBI Official Synonym Full Names: tripartite motif containing 69
• NCBI Official Symbol: TRIM69
• NCBI Official Synonym Symbols: Trif; HSD34; RNF36; HSD-34
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM69; ring finger protein 36; RFP-like domain-containing protein trimless
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM69
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM69
• UniProt Synonym Gene Names: RNF36
• UniProt Entry Name: TRI69_HUMAN

NCBI Description

This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM69: May play a role in apoptosis. Belongs to the TRIM/RBCC family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: cytoplasm; plasma membrane; nuclear speck; nucleus

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: apoptosis; protein ubiquitination