Goat TRIM5/RNF88 Polyclonal Antibody | anti-TRIM5 antibody

Goat anti-TRIM5/RNF88 Antibody

Cat. Num. AAA420059

• Gene Names: TRIM5; RNF88; TRIM5alpha
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TRIM5/RNF88; Polyclonal Antibody; Goat anti-TRIM5/RNF88 Antibody; Goat Anti-TRIM5 / RNF88 (alpha isoform) Antibody; TRIM5; RNF88; tripartite motif-containing 5; TRIM5alpha; tripartite motif protein 5; tripartite motif protein TRIM5; OTTHUMP00000069812; tripartite motif protein TRIM; tripartite motif-containing protein 5 alpha; TRIM5 antibody; RNF88 antibody; tripartite motif-containing 5 antibody; TRIM5alpha antibody; tripartite motif protein 5 antibody; tripartite motif protein TRIM5 antibody; OTTHUMP00000069812 antibody; tripartite motif protein TRIM antibody; tripartite motif-containing protein 5 alpha antibody; TRIM5 / RNF88; anti-TRIM5 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ASGILVNVKEEVTC
• Sequence Length: 493

• Applicable Applications for anti-TRIM5 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Immunohistochemistry: A previous batch was successfully used on Human Tonsil.; Western Blot: Approx 38kDa band observed in Human Plancenta and Human Uterus lysates (calculated MW of 37.7kDa according to NP_149084.1). Recommended concentration: 2-6ug/ml.
• Immunogen: Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1; NP_149083.1; NP_149084.1.
• Epitope: N Terminus
• Note: This antibody is expected to recognise all three human isoforms of this protein.
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 14719418
• NCBI GeneID: 85363
• NCBI Accession #: NP_149023.1; NP_149083.1; NP_149084.1
• NCBI GenBank Nucleotide #: NM_033034.1
• Molecular Weight: 29,677 Da
• NCBI Official Full Name: tripartite motif protein TRIM5 isoform alpha
• NCBI Official Synonym Full Names: tripartite motif containing 5
• NCBI Official Symbol: TRIM5
• NCBI Official Synonym Symbols: RNF88; TRIM5alpha
• NCBI Protein Information: tripartite motif-containing protein 5
• UniProt Protein Name: Tripartite motif-containing protein 5
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM5
• UniProt Synonym Gene Names: RNF88
• UniProt Entry Name: TRIM5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; ligase activity; pattern recognition receptor activity; protein binding; protein binding, bridging; protein homodimerization activity; protein kinase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: activation of innate immune response; activation of NF-kappaB transcription factor; autophagy; defense response to virus; innate immune response; negative regulation of virion penetration into host cell; pattern recognition receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAPKKK cascade; positive regulation of transcription factor activity; regulation of lipopolysaccharide-mediated signaling pathway; regulation of protein localization; viral reproduction