Human Syntenin-1 ELISA Kit | SDCBP elisa kit

Human Syntenin-1 ELISA Kit

Cat. Num. AAA763611

• Gene Names: SDCBP; ST1; MDA9; SYCL; MDA-9; TACIP18
• Reactivity: Human
• Synonyms: Syntenin-1; Human Syntenin-1 ELISA Kit; SDCBP/Syntenin-1/Syndecan-binding protein 1/Scaffold protein Pbp1/Melanoma differentiation-associated protein 9/MDA-9/Pro-TGF-alpha cytoplasmic domain-interacting protein 18/TACIP18/MDA9/SYCL/ST1; SDCBP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SDCBP. No significant cross-reactivity or interference between SDCBP and analogues was observed.
• Sequence Length: 298

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for SDCBP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 56243522
• NCBI GeneID: 6386
• NCBI Accession #: NP_005616.2
• NCBI GenBank Nucleotide #: NM_005625.3
• Molecular Weight: 31,762 Da
• NCBI Official Full Name: syntenin-1 isoform 1
• NCBI Official Synonym Full Names: syndecan binding protein
• NCBI Official Symbol: SDCBP
• NCBI Official Synonym Symbols: ST1; MDA9; SYCL; MDA-9; TACIP18
• NCBI Protein Information: syntenin-1
• UniProt Protein Name: Syntenin-1
• Protein Family: Syntenin
• UniProt Gene Name: SDCBP
• UniProt Synonym Gene Names: MDA9; SYCL; MDA-9; TACIP18
• UniProt Entry Name: SDCB1_HUMAN

NCBI Description

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

syntenin: Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Vesicle; Cytokine; Membrane protein, integral; Cell adhesion; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8q12

Cellular Component: adherens junction; cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum membrane; extracellular space; focal adhesion; interleukin-5 receptor complex; lipid raft; melanosome; membrane; nucleus; plasma membrane

Molecular Function: cell adhesion molecule binding; cytoskeletal adaptor activity; ephrin receptor binding; frizzled binding; glycoprotein binding; growth factor binding; identical protein binding; interleukin-5 receptor binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein homodimerization activity; protein N-terminus binding; syndecan binding

Biological Process: actin cytoskeleton organization and biogenesis; axon guidance; ephrin receptor signaling pathway; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of receptor internalization; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of JNK cascade; positive regulation of phosphorylation; positive regulation of transforming growth factor beta receptor signaling pathway; protein targeting to membrane; Ras protein signal transduction; regulation of mitotic cell cycle; substrate-bound cell migration, cell extension; synaptic transmission