Rabbit anti-Human, Mouse TRIM5 Polyclonal Antibody | anti-TRIM5 antibody

TRIM5 Polyclonal Antibody

Cat. Num. AAA3011446

• Gene Names: TRIM5; RNF88; TRIM5alpha
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TRIM5; Polyclonal Antibody; TRIM5 Polyclonal Antibody; Tripartite motif-containing protein 5; RING finger protein 88; RNF88; anti-TRIM5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIM5 polyclonal antibody detects endogenous levels of TRIM5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 347

• Applicable Applications for anti-TRIM5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human TRIM5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using TRIM5 antibody.)

Related Product Information for anti-TRIM5 antibody

TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family. TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments. Some TRIM proteins, such as TRIM5a, also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm. TRIM5a mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain. The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5a which may form a binding surface for the virus. TRIM5a trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection. The linker region between the coiled-coil and B30.2 domains of TRIM5a is required for this trimerization. TRIM5a blocks infection after the virus has entered the cell.

NCBI and Uniprot Product Information

• NCBI GI #: 283046696
• NCBI GeneID: 85363
• NCBI Accession #: NP_149083
• NCBI GenBank Nucleotide #: NP_149083.2
• UniProt Accession #: Q9C035
• Molecular Weight: ~ 56kDa
• NCBI Official Full Name: tripartite motif-containing protein 5 isoform gamma
• NCBI Official Synonym Full Names: tripartite motif containing 5
• NCBI Official Symbol: TRIM5
• NCBI Official Synonym Symbols: RNF88; TRIM5alpha
• NCBI Protein Information: tripartite motif-containing protein 5
• UniProt Protein Name: Tripartite motif-containing protein 5
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM5
• UniProt Synonym Gene Names: RNF88
• UniProt Entry Name: TRIM5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of MAPKKK cascade; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; defense response to virus; activation of innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; activation of NF-kappaB transcription factor