SDS-Page
(Gel: 6%SDS-PAGE lysate: 40 ¦Œg, Lane: Mouse lung tissue lysate, Primary antibody: 46302£¨ACAP1 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Rabbit anti-Human, Mouse ACAP1 Polyclonal Antibody | anti-ACAP1 antibody
ACAP1 Antibody
Gene Names
ACAP1; CENTB1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Antigen affinity purification
Synonyms
ACAP1; Polyclonal Antibody; ACAP1 Antibody; CENTB1; anti-ACAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous levels of total ACAP1 protein.
Purity/Purification
Antigen affinity purification
Form/Format
In pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
0.7mg/ml (varies by lot)
Sequence Length
2498
Applicable Applications for anti-ACAP1 antibody
Western Blot (WB)
Application Notes
WB: 1:200-1:1000
Immunogen
Synthetic protein corresponding to residues near the C terminal of human ACAP1
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C.
SDS-Page
(Gel: 6%SDS-PAGE lysate: 40 ¦Œg, Lane: Mouse lung tissue lysate, Primary antibody: 46302£¨ACAP1 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
SDS-Page
(Gel: 6%SDS-PAGE lysate: 40 ¦Œg, Lane: Mouse lung tissue lysate, Primary antibody: 46302£¨ACAP1 Antibody) at dilution 1/450 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)
Related Product Information for anti-ACAP1 antibody
ACAP1, also designated Centaurin-¡' 1 (CENTB1 or Cnt-¡'1), is a member of the ADP ribosylation factor family of ARF6 GTPase-activating proteins (GAP). GAPs are important regulators of Arf function by controlling the return of ARF to its inactive state. ACAP1 is related to AGAP1 and ASAP1, and all three proteins are similarly expressed in fibroblast cells such as NIH/3T3. Internalization and recycling of integrin receptors is important in cell adhesion and migration modulation, and once inside a cell, proteins and membranes are transported to the endosome where they are sorted for recycling or degradation. ACAP1 promotes cargo sorting by associating directly to recycling cargo proteins. Preventing this interaction inhibits protein recycling. ACAP1 binds transferrin receptors, promoting their transport to the plasma membrane from the endosome. Akt induced phosphorylation of ACAP1 at Ser 554 regulates ACAP1 interaction to integrin in endosomes, and downregulation of Akt or ACAP1 may inhibit cell migration on Fibronectin.
Product Categories/Family for anti-ACAP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
82 kDa
NCBI Official Full Name
Homo sapiens ArfGAP with coiled-coil, ankyrin repeat and PH domains 1, mRNA
NCBI Official Synonym Full Names
ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
NCBI Official Symbol
ACAP1
NCBI Official Synonym Symbols
CENTB1
NCBI Protein Information
arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
UniProt Protein Name
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
UniProt Gene Name
ACAP1
UniProt Synonym Gene Names
CENTB1; KIAA0050; Cnt-b1
Uniprot Description
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Research Articles on ACAP1
Similar Products
Product Notes
The ACAP1 acap1 (Catalog #AAA9429450) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACAP1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200-1:1000. Researchers should empirically determine the suitability of the ACAP1 acap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
