Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: 231 cells, Jurkat cells, A549 cells, hela cells, 293T cells, Primary antibody: 162369(TRIM45 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Rabbit anti-Human TRIM45 Polyclonal Antibody | anti-TRIM45 antibody

TRIM45 Rabbit pAb

Gene Names
TRIM45; RNF99
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
TRIM45; Polyclonal Antibody; TRIM45 Rabbit pAb; tripartite motif containing 45; RNF99; anti-TRIM45 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-TRIM45 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC: 1:25-1:100
Immunogen
Synthetic peptide of human TRIM45
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Cytoplasm, Nucleus
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: 231 cells, Jurkat cells, A549 cells, hela cells, 293T cells, Primary antibody: 162369(TRIM45 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Western Blot (WB) (Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: 231 cells, Jurkat cells, A549 cells, hela cells, 293T cells, Primary antibody: 162369(TRIM45 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 162369(TRIM45 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 162369(TRIM45 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162369(TRIM45 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 162369(TRIM45 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-TRIM45 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64kDa
NCBI Official Full Name
tripartite motif-containing protein 45 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 45
NCBI Official Symbol
TRIM45
NCBI Official Synonym Symbols
RNF99
NCBI Protein Information
tripartite motif-containing protein 45
UniProt Protein Name
Tripartite motif-containing protein 45
UniProt Gene Name
TRIM45
UniProt Synonym Gene Names
RNF99
UniProt Entry Name
TRI45_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]

Uniprot Description

TRIM45: May act as a transcriptional repressor in mitogen- activated protein kinase signaling pathway. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: cytoplasm; intercellular bridge; nucleoplasm

Molecular Function: zinc ion binding

Research Articles on TRIM45

Similar Products

Product Notes

The TRIM45 trim45 (Catalog #AAA4754911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM45 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM45 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC: 1:25-1:100. Researchers should empirically determine the suitability of the TRIM45 trim45 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM45, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.