Typical Testing Data/Standard Curve (for reference only)
Sheep MAN1A1 ELISA Kit | MAN1A1 elisa kit
Sheep MAN1A1 (Mannosidase Alpha Class 1A Member 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MAN1A1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MAN1A1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MAN1A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MAN1A1. You can calculate the concentration of MAN1A1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq, Jul 2012]
Uniprot Description
MAN1A1: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). Belongs to the glycosyl hydrolase 47 family.
Protein type: Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Endoplasmic reticulum; Calcium-binding; EC 3.2.1.113; Hydrolase
Chromosomal Location of Human Ortholog: 6q22
Cellular Component: Golgi membrane; Golgi apparatus; membrane; endoplasmic reticulum; cytoplasm; ER-Golgi intermediate compartment; integral to membrane
Molecular Function: mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; calcium ion binding; mannosidase activity
Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on MAN1A1
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Product Notes
The Sheep MAN1A1 man1a1 (Catalog #AAA2503594) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503594 ELISA Kit recognizes Sheep MAN1A1. It is sometimes possible for the material contained within the vial of "MAN1A1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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