Rabbit anti-Human TRIM33 Polyclonal Antibody | anti-TRIM33 antibody

TRIM33, CT (TRIM33, KIAA1113, RFG7, TIF1G, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, Transcription intermediary factor 1-gamma, Tripartite motif-containing protein 33) (AP)

Cat. Num. AAA6358286

• Gene Names: TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TRIM33; Polyclonal Antibody; CT (TRIM33; KIAA1113; RFG7; TIF1G; E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; RET-fused gene 7 protein; Transcription intermediary factor 1-gamma; Tripartite motif-containing protein 33) (AP); Tripartite motif-containing protein 33; anti-TRIM33 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIM33.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TRIM33 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1095-1125 in the C-terminal region of human TRIM33, UniProt Accession #Q9UPN9. Species sequence homology: mouse
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS644763 in MDA-MB435 cell line lysates (35ug/lane). This demonstrates that MBS644763 detected the TRIM33 protein (arrow).)

Related Product Information for anti-TRIM33 antibody

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Product Categories/Family for anti-TRIM33 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

1. Bai, X., et al. Cell 142(1):133-143(2010). 2. Howard, P.W., et al. Biochem. Biophys. Res. Commun. 396(3):674-678(2010). 3. Vincent, D.F., et al. PLoS Genet. 5 (7), E1000575 (2009). 4. Dupont, S., et al. Cell 136(1):123-135(2009). 5. Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 74027251
• NCBI GeneID: 51592
• NCBI Accession #: NP_148980.2; NP_056990.3
• NCBI GenBank Nucleotide #: NM_033020.2
• UniProt Accession #: Q9UPN9; O95855; Q5TG72; Q5TG73; Q5TG74; Q9C017; Q9UJ79
• Molecular Weight: 120,541 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM33 isoform beta
• NCBI Official Synonym Full Names: tripartite motif containing 33
• NCBI Official Symbol: TRIM33
• NCBI Official Synonym Symbols: ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM33
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM33
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM33
• UniProt Synonym Gene Names: KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma
• UniProt Entry Name: TRI33_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.

Protein type: Ubiquitin ligase; Transcription, coactivator/corepressor; EC 6.3.2.19; Protein kinase, atypical; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Kinase, protein; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway

Disease: Thyroid Carcinoma, Papillary