Mouse Villin-1 (VIL1) ELISA Kit | VIL1 elisa kit

Mouse Villin-1 (VIL1) ELISA Kit

Cat. Num. AAA7230967

• Gene Names: VIL1; VIL; D2S1471
• Reactivity: Mouse
• Synonyms: Villin-1 (VIL1); Mouse Villin-1 (VIL1) ELISA Kit; VIL1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This VIL-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse VIL-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VIL1 elisa kit

Principle of the assay: VIL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VIL-1 antibody and an VIL-1-HRP conjugate. The assay sample and buffer are incubated together with VIL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VIL-1 concentration since VIL-1 from samples and VIL-1-HRP conjugate compete for the anti-VIL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by VIL-1 from the sample, fewer sites are left to bind VIL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VIL-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for VIL1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 194394237
• NCBI GeneID: 7429
• NCBI Accession #: NP_009058.2
• NCBI GenBank Nucleotide #: NM_007127.2
• Molecular Weight: 46,567 Da
• NCBI Official Full Name: villin-1
• NCBI Official Synonym Full Names: villin 1
• NCBI Official Symbol: VIL1
• NCBI Official Synonym Symbols: VIL; D2S1471
• NCBI Protein Information: villin-1
• UniProt Protein Name: Villin-1
• Protein Family: VIN3-like protein
• UniProt Gene Name: VIL1
• UniProt Synonym Gene Names: VIL
• UniProt Entry Name: VILI_HUMAN

NCBI Description

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]

Uniprot Description

Villin: Epithelial cell-specific Ca(2+)-regulated actin- modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. Monomer. Homodimer; homodimerization is necessary for actin-bundling. Associates with F-actin; phosphorylation at tyrosines residues decreases the association with F-actin. Interacts (phosphorylated at C-terminus tyrosine phosphorylation sites) with PLCG1 (via the SH2 domains). Interacts (phosphorylated form) with PLCG1; the interaction is enhanced by hepatocyte growth factor (HGF). Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes. Belongs to the villin/gelsolin family.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: ruffle; filopodium tip; microvillus; lamellipodium; cytoplasm; actin filament bundle; filopodium

Molecular Function: actin filament binding; identical protein binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein homodimerization activity; caspase inhibitor activity; calcium ion binding

Biological Process: epidermal growth factor receptor signaling pathway; actin filament severing; actin filament depolymerization; actin filament capping; negative regulation of caspase activity; actin nucleation; regulation of cell shape; response to bacterium; epithelial cell differentiation; actin filament polymerization; regulation of actin nucleation; positive regulation of actin filament bundle formation; protein complex assembly; positive regulation of cell migration