Rabbit TRIM30 Polyclonal Antibody | anti-Trim30a antibody

TRIM30, NT (Tripartite motif-containing 30, Rpt-1, Rpt1, down regulatory protein of interleukin 2 receptor)

Cat. Num. AAA620073

• Gene Names: Trim30a; Rpt1; Rpt-1; Trim30
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TRIM30; Polyclonal Antibody; NT (Tripartite motif-containing 30; Rpt-1; Rpt1; down regulatory protein of interleukin 2 receptor); Anti -TRIM30; anti-Trim30a antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human TRIM30.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-Trim30a antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml
• Immunogen: Synthetic peptide corresponding to a 24aa from near the amino terminus of the human TRIM30.
• Positive Control: Human Ovary Tissue Lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of TRIM30 in human ovary tissue lysate with TRIM30 antibody at (A) 1 and (B) 2 ug/ml.)

Related Product Information for anti-Trim30a antibody

TRIM30 belongs to a family of the tripartite motif (TRIM) proteins involved in the regulation of cell proliferation, differentiation, development, oncogenesis, apoptosis and antiviral responses. The TRIM protein family is an expanding family of RING ('really interesting new gene') proteins, also known as RBCC proteins as they contain an RBCC motif, which comprises a RING domain, one or two B-boxes and a predicted coiled-coil region. Studies have shown that some TRIM family members are critical to innate immunity; TRIM5, TRIM19 and TRIM25, for example, have been shown to restrict viral infection. A recent study shows that TRIM30 functions as a negative modulator of the TLR signaling pathway, by targeting TAB2 and TAB3, and contributes to the inhibition of TLR-mediated NFkappa-B activation. The importance of TRIM30 in the attenuation or termination of NFkappa-B activation suggests that targeting of TAB2 and TAB3 by TRIM30 alpha may be a mechanism for modulating many types of immune responses.

Product Categories/Family for anti-Trim30a antibody

Antibodies; Abs to Transcription Factors; TRIM (Tripartite Motif)

NCBI and Uniprot Product Information

• NCBI GI #: 125347379
• NCBI GeneID: 20128
• NCBI Accession #: NP_033125.2
• NCBI GenBank Nucleotide #: NM_009099.2
• UniProt Accession #: P15533; Q99K55; Q99PQ7; Q99PQ8; Q99PQ9; Q3UUN0; Q3UV85; Q3UZ29
• Molecular Weight: 57,330 Da
• NCBI Official Full Name: tripartite motif-containing protein 30
• NCBI Official Synonym Full Names: tripartite motif-containing 30A
• NCBI Official Symbol: Trim30a
• NCBI Official Synonym Symbols: Rpt1; Rpt-1; Trim30
• NCBI Protein Information: tripartite motif-containing protein 30; OTTMUSP00000020742; tripartite motif protein 30; tripartite motif-containing 30; regulatory protein, T lymphocyte 1; down regulatory protein of interleukin-2 receptor
• UniProt Protein Name: Tripartite motif-containing protein 30
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: Trim30
• UniProt Synonym Gene Names: Rpt-1; Rpt1
• UniProt Entry Name: TRI30_MOUSE

Uniprot Description

TRIM30A: Trans-acting factor that regulates gene expression of interleukin 2 receptor alpha chain. May affect IL2R-alpha expression through cis-acting negative regulatory elements or through competition with proteins that bind to enhancer or activator sequences. Negatively regulates Toll-like receptor (TLR)-mediated activation of NFKB by promoting degradation of TAB2 and TAB3 and preventing TRAF6 autoubiquitination. Negatively regulates production of reactive oxygen species (ROS) which inhibits activation of the NLRP3 inflammasome complex. This, in turn, regulates activation of CASP1 and subsequent cleavage of IL1B and IL18. No activity detected against a range of retroviruses including a number of lentiviruses, gammaretroviruses and betaretroviruses

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: DNA binding; zinc ion binding; metal ion binding

Biological Process: protein autoubiquitination; regulation of transcription, DNA-dependent; positive regulation of virion penetration into host cell; transcription, DNA-dependent; positive regulation of protein catabolic process; negative regulation of interleukin-6 production; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production