Goat anti-Human TRIM2 Polyclonal Antibody | anti-TRIM2 antibody

TRIM2, CT (Tripartite Motif-containing Protein 2, RING Finger Protein 86, KIAA0517, RNF86)

Cat. Num. AAA646176

• Gene Names: TRIM2; RNF86
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TRIM2; Polyclonal Antibody; CT (Tripartite Motif-containing Protein 2; RING Finger Protein 86; KIAA0517; RNF86); Anti -TRIM2; anti-TRIM2 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human TRIM2. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-TRIM2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:16,000; Western Blot: 0.5-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 6.25ug/ml
• Immunogen: Synthetic peptide corresponding to DSGNHCFKVYRYLQ of human TRIM2 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex: Formalin- Fixed, Paraffin-Embedded (FFPE))

Chemiluminescence

(TRIM2 antibody (0.5ug/ml) staining of Rat Brain lysate (35ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-TRIM2 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Product Categories/Family for anti-TRIM2 antibody

Antibodies; Abs to Transcription Factors; TRIM (Tripartite Motif)

NCBI and Uniprot Product Information

• NCBI GI #: 194248081
• NCBI GeneID: 23321
• NCBI Accession #: NP_001123539.1
• NCBI GenBank Nucleotide #: NM_001130067.1
• UniProt Accession #: Q9C040; O60272; Q9BSI9; Q9UFZ1; D3DP09
• Molecular Weight: 81,530 Da
• NCBI Official Full Name: tripartite motif-containing protein 2 isoform 2
• NCBI Official Synonym Full Names: tripartite motif containing 2
• NCBI Official Symbol: TRIM2
• NCBI Official Synonym Symbols: RNF86
• NCBI Protein Information: tripartite motif-containing protein 2; RING finger protein 86; tripartite motif protein 2; tripartite motif-containing 2; tripartite motif protein TRIM2; E3 ubiquitin-protein ligase TRIM2
• UniProt Protein Name: Tripartite motif-containing protein 2
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM2
• UniProt Synonym Gene Names: KIAA0517; RNF86
• UniProt Entry Name: TRIM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11

By similarity. Plays a neuroprotective function

By similarity. May play a role in neuronal rapid ischemic tolerance

By similarity.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with myosin V; myosin V may not be a substrate for ubiquitination

By similarity. Interacts with NEFL

By similarity. Interacts with phosphorylated BCL2L11. Ref.7

Subcellular location: Cytoplasm

By similarity.

Domain: The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades

By similarity.

Post-translational modification: RING-type zinc finger-dependent and UBE2D1-dependent autoubiquitination

By similarity.

Sequence similarities: Belongs to the TRIM/RBCC family.Contains 1 B box-type zinc finger.Contains 1 filamin repeat.Contains 6 NHL repeats.Contains 1 RING-type zinc finger.

Sequence caution: The sequence AAG53472.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAA25443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.