Western Blot (WB)
(Western Blot analysis of MBS615262.)
Rabbit TRIM5d Polyclonal Antibody
TRIM5d (Tripartite Motif Protein 5d)
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
TRIM5d; Polyclonal Antibody; TRIM5d (Tripartite Motif Protein 5d); Anti -TRIM5d (Tripartite Motif Protein 5d); anti-TRIM5d antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TRIM5d. Species Crossreactivity: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Applicable Applications for anti-TRIM5d antibody
Western Blot (WB)
Application Notes
Can be used for detection of TRIM5d by Western Blot at 1-2ug/ml. Mouse brain cell lysate can be used as a positive control and a band at approximately 25kD can be detected.
Immunogen
Synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5d (Genbank accession No. NP_149084)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis of MBS615262.)
Western Blot (WB)
(Western Blot analysis of MBS615262.)
Related Product Information for anti-TRIM5d antibody
TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5a variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV- 1 (2,3), albeit at differing efficiencies. The TRIM5d isoform has been shown to colocalize with the topoisomerase Iinteracting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5 d also exhibits autoubiquitination activity in a RING finger- and UbcH5Bdependent manner (4).
Product Categories/Family for anti-TRIM5d antibody
Similar Products
Product Notes
The TRIM5d (Catalog #AAA615262) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM5d (Tripartite Motif Protein 5d) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM5d can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Can be used for detection of TRIM5d by Western Blot at 1-2ug/ml. Mouse brain cell lysate can be used as a positive control and a band at approximately 25kD can be detected. Researchers should empirically determine the suitability of the TRIM5d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM5d, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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