Human Lymphocyte activation gene 3 protein ELISA Kit | LAG3 elisa kit

Human Lymphocyte activation gene 3 protein ELISA Kit

Cat. Num. AAA762098

• Gene Names: LAG3; CD223
• Reactivity: Human
• Synonyms: Lymphocyte activation gene 3 protein; Human Lymphocyte activation gene 3 protein ELISA Kit; LAG3 (Lymphocyte Activation Gene 3)/LAG-3/CD223/CD223 antigen/FDC/lymphocyte activation gene 3 protein/lymphocyte-activation gene 3/Protein FDC; LAG3 elisa kit

• Reactivity: Human
• Sequence Length: 525

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for LAG3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human LAG3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human LAG3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LAG3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman LAG3can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277.4
• NCBI GenBank Nucleotide #: NM_002286.5
• Molecular Weight: 39,103 Da
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte activating 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN

NCBI Description

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: external side of plasma membrane; integral to membrane

Molecular Function: antigen binding; MHC class II protein binding; transmembrane receptor activity

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; negative regulation of T cell activation; positive regulation of natural killer cell mediated cytotoxicity