Rabbit anti-Human, Mouse TRIM24 / TIF1 Polyclonal Antibody | anti-TRIM24 antibody

Rabbit Polyclonal (IgG) to Human TRIM24 / TIF1

Cat. Num. AAA247305

• Gene Names: TRIM24; PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRIM24 / TIF1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TRIM24 / TIF1; Anti-TRIM24 / TIF1 Antibody (aa1001-1050) IHC-plus; TRIM24; TF1A; TIF1; TIF1A; Tripartite motif-containing 24; TIF1ALPHA; PTC6; RNF82; TIF1-alpha; Tripartite motif containing 24; HTIF1; RING finger protein 82; Human TRIM24; anti-TRIM24 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIM24 Antibody detects endogenous levels of total TRIM24 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1016

• Applicable Applications for anti-TRIM24 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TRIM24 (aa1001-1050).
• Immunogen: TRIM24 / TIF1 antibody was raised against synthetic peptide from human TRIM24 (aa1001-1050).
• Antigen Modification: aa1001-1050
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-TRIM24 / TIF1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from COLO cells, using TRIM24 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-TRIM24 antibody

TIF1alpha is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily. TIF1alpha contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TIF1alpha acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TIF1alpha associates tightly with euchromatin, where its silencing activity appears to result chiefly from histone deacetylation.

Product Categories/Family for anti-TRIM24 antibody

Family: Tripartite Motif; Subfamily: TIF1

NCBI and Uniprot Product Information

• NCBI GI #: 47419909
• NCBI GeneID: 8805
• NCBI Accession #: NP_003843.3
• NCBI GenBank Nucleotide #: NM_003852.3
• UniProt Accession #: O15164; O95854; A4D1R7; A4D1R8
• Molecular Weight: 113,018 Da
• NCBI Official Full Name: transcription intermediary factor 1-alpha isoform b
• NCBI Official Synonym Full Names: tripartite motif containing 24
• NCBI Official Symbol: TRIM24
• NCBI Official Synonym Symbols: PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
• NCBI Protein Information: transcription intermediary factor 1-alpha; TIF1-alpha; RING finger protein 82; tripartite motif-containing 24; E3 ubiquitin-protein ligase TRIM24; transcriptional intermediary factor 1
• UniProt Protein Name: Transcription intermediary factor 1-alpha
• Protein Family: Transcription intermediary factor
• UniProt Gene Name: TRIM24
• UniProt Synonym Gene Names: RNF82; TIF1; TIF1A; TIF1-alpha
• UniProt Entry Name: TIF1A_HUMAN

NCBI Description

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM24: an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55. Belongs to a family of nuclear proteins that includes TIF1b and TIF1g. TIF1 proteins are characterized by an N-terminal region that contains a RING finger-B boxes-coiled coil (RBCC) motif, a poorly conserved central region, a C-terminal region that contains a PHD finger, and a bromodomain. Interacts with the heterochromatin-associated proteins HP1a, MOD1 (HP1b), and MOD2 (HP1g) and with the transcriptional repression domain KRAB, which is present in many Kruppel-type (C2H2) zinc finger proteins. May act as a repressor through the formation of transcriptionally inactive heterochromatin. Two splice variant isoforms have been described.

Protein type: Ubiquitin conjugating system; Transcription, coactivator/corepressor; EC 6.3.2.-; Tumor suppressor; Kinase, protein; Protein kinase, atypical; Nuclear receptor co-regulator; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 7q32-q34

Cellular Component: nucleoplasm; perichromatin fibrils; cytosol; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; zinc ion binding; sequence-specific DNA binding; p53 binding; transcription coactivator activity; ubiquitin-protein ligase activity; estrogen response element binding; chromatin binding; methylated histone residue binding; receptor binding; protein kinase activity; ligase activity

Biological Process: transcription from RNA polymerase II promoter; calcium ion homeostasis; regulation of apoptosis; response to peptide hormone stimulus; negative regulation of cell proliferation; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; regulation of protein stability; protein ubiquitination; protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Thyroid Carcinoma, Papillary