Rabbit anti-Human TRIM22 Polyclonal Antibody | anti-TRIM22 antibody

TRIM22, NT (TRIM22, RNF94, STAF50, E3 ubiquitin-protein ligase TRIM22, 50kD-stimulated trans-acting factor, RING finger protein 94, Staf-50, Tripartite motif-containing protein 22) (FITC)

Cat. Num. AAA6358190

• Gene Names: TRIM22; RNF94; STAF50; GPSTAF50
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRIM22; Polyclonal Antibody; NT (TRIM22; RNF94; STAF50; E3 ubiquitin-protein ligase TRIM22; 50kD-stimulated trans-acting factor; RING finger protein 94; Staf-50; Tripartite motif-containing protein 22) (FITC); Tripartite motif-containing protein 22; anti-TRIM22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 498

• Applicable Applications for anti-TRIM22 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TRIM22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the N-terminal region of human TRIM22.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRIM22 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the TRIM22 antibody detected the TRIM22 protein (arrow).)

Related Product Information for anti-TRIM22 antibody

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects.

Product Categories/Family for anti-TRIM22 antibody

Antibodies; Transcription Factors; TRIM (Tripartite Motif)

References

Kajaste-Rudnitski, A., et al. Amino Acids 39(1):1-9(2010). Petersson, J., et al. Exp. Cell Res. 316(4):568-579(2010). Ovsyannikova, I.G., et al. Hum. Genet. 127(2):207-221(2010). Ovsyannikova, I.G., et al. J. Infect. Dis. 201(2):207-213(2010). Gao, B., et al. Hepatology 50(2):424-433(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 117938316
• NCBI GeneID: 10346
• NCBI Accession #: NP_006065.2
• NCBI GenBank Nucleotide #: NP_006065.2
• UniProt Accession #: Q8IYM9
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM22 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 22
• NCBI Official Symbol: TRIM22
• NCBI Official Synonym Symbols: RNF94; STAF50; GPSTAF50
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM22
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM22
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM22
• UniProt Synonym Gene Names: RNF94; STAF50
• UniProt Entry Name: TRI22_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

TRIM22: Interferon-induced antiviral protein involved in cell innate immunity. The antiviral activity could in part be mediated by TRIM22-dependent ubiquitination of viral proteins. Plays a role in restricting the replication of HIV-1, encephalomyocarditis virus (EMCV) and hepatitis B virus (HBV). Acts as a transcriptional repressor of HBV core promoter. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Transcription, coactivator/corepressor; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: nucleoplasm; Cajal body; cytoplasm; nuclear speck; cytosol; nucleus

Molecular Function: zinc ion binding; transcription factor activity; transcription corepressor activity; ligase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; transcription, DNA-dependent; viral reproduction; response to virus; cytokine and chemokine mediated signaling pathway; protein ubiquitination; positive regulation of transcription factor activity; immune response; defense response to virus; activation of NF-kappaB transcription factor