Rabbit anti-Human, Mouse TRAF4 Polyclonal Antibody | anti-Traf4 antibody

Anti-TRAF4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100757

• Gene Names: TRAF4; CART1; MLN62; RNF83
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: TRAF4; Polyclonal Antibody; Anti-TRAF4 Antibody; Rabbit Polyclonal; Traf4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CART1 Antibody; Anti-MLN62 Antibody; Anti-RNF83 Antibody; anti-Traf4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TRAF4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Traf4 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: IHC-P: 1:250-1:1000; ICC/IF: 1:300-1:10,000; IP: 4-8uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human Traf4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Traf4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining TRAF4 in human kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining TRAF4 in human lung cancer with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining TRAF4 in mouse kidney with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of TRAF4 in U2OS and HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TRAF4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(TRAF4 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate4 uL anti-TRAF4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-TRAF4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)

Related Product Information for anti-Traf4 antibody

traf4a mRNA is expressed in the developing eye vesicle over the period of optic cup morphogenesis, and Traf4a loss leads to disrupted evagination and elongation of the eye vesicles, and aberrant organization and apico-basal polarity of the eye epithelium. Traf4 acts in a TNF receptor-and Jun N-terminal protein kinase (JNK)-independent manner to fine-tune the assembly of adherens junctions in the invaginating mesodermal cells. Tumor necrosis factor receptor associated factor 4 (Traf4) mRNA expression is upregulated in various breast tumors and tumor cell lines.

Product Categories/Family for anti-Traf4 antibody

IL17 signaling pathway

NCBI and Uniprot Product Information

• NCBI GI #: 22027622
• NCBI GeneID: 9618
• NCBI Accession #: NP_004286.2
• NCBI GenBank Nucleotide #: NM_004295.3
• UniProt Accession #: Q9BUZ4; O75615; Q14848; Q2KJU4; Q2PJN8
• Molecular Weight: 53,543 Da
• NCBI Official Full Name: TNF receptor-associated factor 4
• NCBI Official Synonym Full Names: TNF receptor-associated factor 4
• NCBI Official Symbol: TRAF4
• NCBI Official Synonym Symbols: CART1; MLN62; RNF83
• NCBI Protein Information: TNF receptor-associated factor 4; MLN 62; malignant 62; RING finger protein 83; metastatic lymph node gene 62 protein; tumor necrosis receptor-associated factor 4A; cysteine-rich domain associated with ring and TRAF domain; cysteine-rich domain associated
• UniProt Protein Name: TNF receptor-associated factor 4
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF4
• UniProt Synonym Gene Names: CART1; MLN62; RNF83; MLN 62
• UniProt Entry Name: TRAF4_HUMAN

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: tight junction; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; WW domain binding; tumor necrosis factor receptor binding; protein kinase binding

Biological Process: regulation of apoptosis; apoptosis; positive regulation of protein kinase activity; respiratory tube development; activation of NF-kappaB-inducing kinase; positive regulation of JNK cascade; protein ubiquitination; respiratory gaseous exchange; signal transduction