Rabbit anti-Human TRAF4 Polyclonal Antibody | anti-TRAF4 antibody

TRAF4, CT (TRAF4, CART1, MLN62, RNF83, TNF receptor-associated factor 4, Cysteine-rich domain associated with RING and Traf domains protein 1, Metastatic lymph node gene 62 protein, RING finger protein 83)

Cat. Num. AAA6005907

• Gene Names: TRAF4; CART1; MLN62; RNF83
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TRAF4; Polyclonal Antibody; CT (TRAF4; CART1; MLN62; RNF83; TNF receptor-associated factor 4; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; RING finger protein 83); Anti -TRAF4; anti-TRAF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TRAF4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: TRAF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-455 amino acids from the C-terminal region of human TRAF4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TRAF4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TRAF4 antibody detected the TRAF4 protein (arrow).)

Related Product Information for anti-TRAF4 antibody

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined.

Product Categories/Family for anti-TRAF4 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 22027622
• NCBI GeneID: 9618
• NCBI Accession #: NP_004286.2
• NCBI GenBank Nucleotide #: NM_004295.3
• UniProt Accession #: Q9BUZ4; O75615; Q14848; Q2KJU4; Q2PJN8
• Molecular Weight: 53,543 Da
• NCBI Official Full Name: TNF receptor-associated factor 4
• NCBI Official Synonym Full Names: TNF receptor-associated factor 4
• NCBI Official Symbol: TRAF4
• NCBI Official Synonym Symbols: CART1; MLN62; RNF83
• NCBI Protein Information: TNF receptor-associated factor 4; MLN 62; malignant 62; RING finger protein 83; metastatic lymph node gene 62 protein; tumor necrosis receptor-associated factor 4A; cysteine-rich domain associated with ring and TRAF domain; cysteine-rich domain associated with RING and Traf domains protein 1
• UniProt Protein Name: TNF receptor-associated factor 4
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF4
• UniProt Synonym Gene Names: CART1; MLN62; RNF83; MLN 62
• UniProt Entry Name: TRAF4_HUMAN

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; DNA-binding

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: cytoskeleton; tight junction; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin protein ligase binding; thioesterase binding; ubiquitin-protein ligase activity; WW domain binding; protein kinase binding; tumor necrosis factor receptor binding

Biological Process: regulation of apoptosis; apoptosis; respiratory tube development; positive regulation of protein kinase activity; activation of NF-kappaB-inducing kinase; positive regulation of JNK cascade; protein ubiquitination; respiratory gaseous exchange; signal transduction