Rabbit Glucocorticoid Receptor Monoclonal Antibody | anti-NR3C1 antibody

Glucocorticoid Receptor Rabbit mAb

Cat. Num. AAA4752770

• Gene Names: Nr3c1; GR; Grl1; Grl-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: Glucocorticoid Receptor; Monoclonal Antibody; Glucocorticoid Receptor Rabbit mAb; Glucocorticoid receptor; GR; GCR; GRL; GCCR; GCRST; anti-NR3C1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R04-8C8
• Specificity: Expressed in spleen, kidney and liver. Isoform 3: Expressed at highest level in spleen with lesser amounts in kidney and liver.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-NR3C1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:200; ICC/IF: 1:50; FC/FACS: 1:50
• Immunogen: Recombinant protein of mouse Glucocorticoid Receptor
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of Glucocorticoid Receptor in K562,3T3,Hela cell lysates using Glucocorticoid Receptor Rabbit mAb(1:1000 diluted). Predicted band size:86kDa. Observed band size: 94,91kDa.)

Related Product Information for anti-NR3C1 antibody

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:10678832). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (PubMed:15037546).

NCBI and Uniprot Product Information

• NCBI GI #: 121073
• NCBI GeneID: 14815
• UniProt Accession #: P06537; Q61628; Q61629; E0ZPU5
• Molecular Weight: 81,888 Da
• NCBI Official Full Name: Glucocorticoid receptor
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3, group C, member 1
• NCBI Official Symbol: Nr3c1
• NCBI Official Synonym Symbols: GR; Grl1; Grl-1
• NCBI Protein Information: glucocorticoid receptor
• UniProt Protein Name: Glucocorticoid receptor
• Protein Family: Glucocorticoid receptor
• UniProt Gene Name: Nr3c1
• UniProt Synonym Gene Names: Grl; Grl1; GR

Uniprot Description

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:10678832). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (PubMed:15037546).