Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peptidase inhibitor 16 (PI16) Standard Curve.)
Human Peptidase Inhibitor 16 (PI16) ELISA Kit | PI16 elisa kit
Human Peptidase Inhibitor 16 (PI16) ELISA Kit
Gene Names
PI16; CD364; PSPBP; CRISP9; MSMBBP
Reactivity
Human
Synonyms
Peptidase Inhibitor 16 (PI16); Human Peptidase Inhibitor 16 (PI16) ELISA Kit; PI16; CRISP9; DKFZp586B1817; MGC45378; MSMBBP; PSPBP; PSP94-binding protein; microseminoprotein; beta-binding protein; protease inhibitor 16; PI16 elisa kit
Reactivity
Human
Specificity
Human Peptidase inhibitor 16 (PI16) ELISA Kit has high sensitivity and excellent specificity for detection of Human PI16. No significant cross-reactivity or interference between Human PI16 and analogues was observed.
Sequence Length
463
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peptidase inhibitor 16 (PI16) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Peptidase inhibitor 16 (PI16) Standard Curve.)
Related Product Information for PI16 elisa kit
Description: This Human Peptidase inhibitor 16 (PI16) ELISA Kit employs a two-site sandwich ELISA to quantitate PI16 in samples. An antibody specific for PI16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PI16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PI16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PI16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PI16 is a putative serine protease inhibitor. PI16 may serve as a marker following prostatectomy for prostate cancer. This protein binds with high affinity to PSP94. PI16 expression is enhanced after serum stimulation of primary cardiomyocytes and is rapidly secreted into the conditioned medium of cardiomyocytes. PI16 is overexpressed in the hypertrophic and failing heart and inhibits cardiomyocyte growth. Suppression of endogenous PI16 expression in primary cardiomyocytes significantly enhances cardiomyocyte size. Transgenic mice overexpressing PI16 in a cardiomyocyte-specific manner show normal cardiac function but have smaller hearts with hypotrophic cardiomyocytes.
Background: PI16 is a putative serine protease inhibitor. PI16 may serve as a marker following prostatectomy for prostate cancer. This protein binds with high affinity to PSP94. PI16 expression is enhanced after serum stimulation of primary cardiomyocytes and is rapidly secreted into the conditioned medium of cardiomyocytes. PI16 is overexpressed in the hypertrophic and failing heart and inhibits cardiomyocyte growth. Suppression of endogenous PI16 expression in primary cardiomyocytes significantly enhances cardiomyocyte size. Transgenic mice overexpressing PI16 in a cardiomyocyte-specific manner show normal cardiac function but have smaller hearts with hypotrophic cardiomyocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptidase inhibitor 16
NCBI Official Synonym Full Names
peptidase inhibitor 16
NCBI Official Symbol
PI16
NCBI Official Synonym Symbols
CD364; PSPBP; CRISP9; MSMBBP
NCBI Protein Information
peptidase inhibitor 16
UniProt Protein Name
Peptidase inhibitor 16
Protein Family
UniProt Gene Name
PI16
UniProt Synonym Gene Names
CRISP9; PSPBP; PI-16; CRISP-9
UniProt Entry Name
PI16_HUMAN
Uniprot Description
PI16: Putative serine protease inhibitor. Belongs to the CRISP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.2
Cellular Component: extracellular space; integral to membrane
Molecular Function: protease inhibitor activity
Biological Process: negative regulation of peptidase activity
Research Articles on PI16
Similar Products
Product Notes
The Human PI16 pi16 (Catalog #AAA9714884) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714884 ELISA Kit recognizes Human PI16. It is sometimes possible for the material contained within the vial of "Peptidase Inhibitor 16 (PI16), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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