Rabbit anti-Human TPTEa Polyclonal Antibody

TPTEa, NT (TPTE, Putative tyrosine-protein phosphatase TPTE, Cancer/testis antigen 44, Transmembrane phosphatase with tensin homology, Tumor antigen BJ-HCC-5)

Cat. Num. AAA6006340

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TPTEa; Polyclonal Antibody; NT (TPTE; Putative tyrosine-protein phosphatase TPTE; Cancer/testis antigen 44; Transmembrane phosphatase with tensin homology; Tumor antigen BJ-HCC-5); Anti -TPTEa; anti-TPTEa antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TPTEa antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: TPTEa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51~80 amino acids from the N-terminal region of human TPTEa.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TPTEa Antibody (E57) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TPTEa antibody detected the TPTEa protein (arrow).)

Immunohistochemistry (IHC)

(TPTEa Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TPTEa Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TPTEa antibody

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Product Categories/Family for anti-TPTEa antibody

Antibodies; Abs to Transmembrane Proteins