Rabbit anti-Human MRGPRX2 Polyclonal Antibody | anti-MRGPRX2 antibody

MRGPRX2, CT (MRGPRX2, MRGX2, Mas-related G-protein coupled receptor member X2) (APC)

Cat. Num. AAA6322099

• Gene Names: MRGPRX2; MGRG3; MRGX2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MRGPRX2; Polyclonal Antibody; CT (MRGPRX2; MRGX2; Mas-related G-protein coupled receptor member X2) (APC); Mas-related G-protein coupled receptor member X2; anti-MRGPRX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MRGPRX2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Concentration: 0.5mg/mL (varies by lot)

• Applicable Applications for anti-MRGPRX2 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 287-315 in the C-terminal region of human MRGPRX2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS6009617. This demonstrates that MBS6009617 detected the MRGPRX2 protein (arrow).)

Related Product Information for anti-MRGPRX2 antibody

MRGPRX2 is the orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin-stimulated cAMP levels, suggesting that this receptor is G(q)-coupled.

Product Categories/Family for anti-MRGPRX2 antibody

Antibodies; G Protein Receptors

References

1. Gembardt, F., et al. Mol. Cell. Biochem. 319 (1-2), 115-123 (2008). 2. Yang, S., et al. Gene 352, 30-35 (2005). 3. Robas, N., et al. J. Biol. Chem. 278(45):44400-44404(2003). 4. Takeda, S., et al. FEBS Lett. 520 (1-3), 97-101 (2002). 5. Dong, X., et al. Cell 106(5):619-632(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 16876451
• NCBI GeneID: 117194
• NCBI Accession #: NP_473371.1
• NCBI GenBank Nucleotide #: NM_054030.2
• UniProt Accession #: Q96LB1; Q4QXW4; Q4QXW7; Q4QXX0; Q4QXX2; Q4QXX3; Q4QXX4; Q4QXX6; Q4QXX7; B5B0C7
• Molecular Weight: 37,099 Da
• NCBI Official Full Name: mas-related G-protein coupled receptor member X2
• NCBI Official Synonym Full Names: MAS-related GPR, member X2
• NCBI Official Symbol: MRGPRX2
• NCBI Official Synonym Symbols: MGRG3; MRGX2
• NCBI Protein Information: mas-related G-protein coupled receptor member X2; G protein-coupled receptor MRGX2; Mas-related G protein-coupled receptor G3
• UniProt Protein Name: Mas-related G-protein coupled receptor member X2
• Protein Family: Mas-related G-protein coupled receptor
• UniProt Gene Name: MRGPRX2
• UniProt Synonym Gene Names: MRGX2
• UniProt Entry Name: MRGX2_HUMAN

Uniprot Description

MRGPRX2: Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Cortistatin-14 seems to be a high potency ligand at this receptor. Cortistatin has several biological functions including roles in sleep regulation locomotor activity, and cortical function. In receptor-expressing cells, cortistatin-stimulated increases in intracellular Ca(2+) but had no effect on basal or forskolin- stimulated cAMP levels, suggesting that this receptor is G(q)- coupled. Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding

Biological Process: positive regulation of cytokinesis; G-protein coupled receptor protein signaling pathway; sensory perception of pain; sleep