Rabbit anti-Human TPTEa Polyclonal Antibody | anti-TPTEa antibody

TPTEa, NT (TPTE, Putative tyrosine-protein phosphatase TPTE, Cancer/testis antigen 44, Transmembrane phosphatase with tensin homology, Tumor antigen BJ-HCC-5) (PE)

Cat. Num. AAA6357790

• Gene Names: TPTE; CT44; PTEN2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TPTEa; Polyclonal Antibody; NT (TPTE; Putative tyrosine-protein phosphatase TPTE; Cancer/testis antigen 44; Transmembrane phosphatase with tensin homology; Tumor antigen BJ-HCC-5) (PE); TPTE; Tumor antigen BJ-HCC-5; anti-TPTEa antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TPTEa antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TPTEa antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51~80 amino acids from the N-terminal region of human TPTEa.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TPTEa Antibody (E57) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TPTEa antibody detected the TPTEa protein (arrow).)

Immunohistochemistry (IHC)

(TPTEa Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TPTEa Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TPTEa antibody

Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.

Product Categories/Family for anti-TPTEa antibody

Antibodies; Transmembrane Proteins

References

Hattori, M., et al., Nature 405(6784):311-319 (2000). Chen, H., et al., Hum. Genet. 105(5):399-409 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 109689707
• NCBI GeneID: 7179
• NCBI Accession #: NP_954870.2
• NCBI GenBank Nucleotide #: NM_199261.3
• UniProt Accession #: P56180; Q6XPS4; Q6XPS5; Q71JA8; Q8NCS8; B2RAP7; C9J6D6; C9JKK8
• Molecular Weight: 49,344 Da
• NCBI Official Full Name: putative tyrosine-protein phosphatase TPTE isoform alpha
• NCBI Official Synonym Full Names: transmembrane phosphatase with tensin homology
• NCBI Official Symbol: TPTE
• NCBI Official Synonym Symbols: CT44; PTEN2
• NCBI Protein Information: putative tyrosine-protein phosphatase TPTE; tumor antigen BJ-HCC-5; cancer/testis antigen 44; PTEN-related tyrosine phosphatase; tensin, putative protein-tyrosine phosphatase
• UniProt Protein Name: Putative tyrosine-protein phosphatase TPTE
• UniProt Gene Name: TPTE
• UniProt Synonym Gene Names: CT44
• UniProt Entry Name: TPTE_HUMAN

NCBI Description

This gene encodes a PTEN-related tyrosine phosphatase which may play a role in the signal transduction pathways of the endocrine or spermatogenic function of the testis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

TPTE: Could be involved in signal transduction. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Membrane protein, multi-pass; Receptor protein phosphatase, tyrosine; Membrane protein, integral; EC 3.1.3.48; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 21p11

Cellular Component: integral to membrane

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; ion channel activity; protein tyrosine phosphatase activity

Biological Process: signal transduction; protein amino acid dephosphorylation