TPMT antibody pair

TPMT Antibody Pair

Cat. Num. AAA7041710

• Gene Names: TPMT; TPMTD
• Reactivity: Rat, Mouse, Horse, Pig, Chicken, Rabbit, Goat, Sheep
• Applications: ELISA
• Synonyms: TPMT; Polyclonal Antibody Pair; TPMT Antibody Pair; Thiopurine S-methyltransferase; Thiopurine methyltransferase; TPMT antibody pair

• Host: Capture: Rabbit; Detection: Rabbit
• Reactivity: Rat, Mouse, Horse, Pig, Chicken, Rabbit, Goat, Sheep
• Clonality: Polyclonal
• Isotype: Capture: IgG; Detection: IgG
• Form/Format: Liquid
• Concentration: The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
• Sequence Length: 245

• Applicable Applications for TPMT antibody pair: Sandwich ELISA (EIA)
• Application Notes: We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.25ug/ml.
• Immunogen Species: Human
• Conjugation: Capture: Non-conjugated; Detection: Biotin conjugated
• Recommended Standard: Hela cell; MDA-MB-435 cell; (Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
• Storage Buffer: Capture: 50% Glycerol, 0.01M PBS, PH 7.4; Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Other Note: Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

• NCBI GI #: 4507653
• NCBI GeneID: 7172
• NCBI Accession #: NP_000358.1
• NCBI GenBank Nucleotide #: NM_000367.3
• Molecular Weight: 28,180 Da
• NCBI Official Full Name: thiopurine S-methyltransferase
• NCBI Official Synonym Full Names: thiopurine S-methyltransferase
• NCBI Official Symbol: TPMT
• NCBI Official Synonym Symbols: TPMTD
• NCBI Protein Information: thiopurine S-methyltransferase
• UniProt Protein Name: Thiopurine S-methyltransferase
• UniProt Gene Name: TPMT
• UniProt Entry Name: TPMT_HUMAN

NCBI Description

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

Uniprot Description

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family.

Protein type: EC 2.1.1.67; Methyltransferase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytosol

Molecular Function: thiopurine S-methyltransferase activity

Biological Process: methylation; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Disease: Thiopurine S-methyltransferase Deficiency