Rabbit anti-Human CASQ2 Polyclonal Antibody | anti-CASQ2 antibody

CASQ2, ID (CASQ2, Calsequestrin-2, Calsequestrin, cardiac muscle isoform) (FITC)

Cat. Num. AAA6280961

• Gene Names: CASQ2; PDIB2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CASQ2; Polyclonal Antibody; ID (CASQ2; Calsequestrin-2; Calsequestrin; cardiac muscle isoform) (FITC); cardiac muscle isoform; anti-CASQ2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CASQ2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CASQ2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-107 amino acids from the Central region of human CASQ2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CASQ2 Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the CASQ2 antibody detected the CASQ2 protein (arrow).)

Related Product Information for anti-CASQ2 antibody

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq].

Product Categories/Family for anti-CASQ2 antibody

Antibodies; Cardiac Markers

References

Kirchhefer, U., et al. J. Mol. Cell. Cardiol. 49(1):95-105(2010). Wong, C.H., et al. Forensic Sci. Int. 192 (1-3), 53-55 (2009): Liu, J., et al. Am. J. Physiol., Cell Physiol. 297 (1), C152-C159 (2009): Hayashi, M., et al. Circulation 119(18):2426-2434(2009). Wei, L., et al. Cell Calcium 45(5):474-484(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 119395727
• NCBI GeneID: 845
• NCBI Accession #: NP_001223.2
• NCBI GenBank Nucleotide #: NM_001232.3
• UniProt Accession #: O14958; Q5T1D2; Q8TBW8; B2R7M6; B4DIB0
• Molecular Weight: 38,269 Da
• NCBI Official Full Name: calsequestrin-2
• NCBI Official Synonym Full Names: calsequestrin 2 (cardiac muscle)
• NCBI Official Symbol: CASQ2
• NCBI Official Synonym Symbols: PDIB2
• NCBI Protein Information: calsequestrin-2; calsequestrin 2, fast-twitch, cardiac muscle; calsequestrin, cardiac muscle isoform
• UniProt Protein Name: Calsequestrin-2
• Protein Family: Calsequestrin
• UniProt Gene Name: CASQ2
• UniProt Entry Name: CASQ2_HUMAN

NCBI Description

The protein encoded by this gene specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. The protein is a calcium binding protein that stores calcium for muscle function. Mutations in this gene cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest. [provided by RefSeq, Jul 2008]

Uniprot Description

calsequestrin 2: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+) ions. Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2); also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. Belongs to the calsequestrin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: sarcoplasmic reticulum membrane; sarcoplasmic reticulum lumen; sarcoplasmic reticulum; cytoplasm; junctional membrane complex; voltage-gated calcium channel complex; intracellular; Z disc

Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; calcium-dependent protein binding

Biological Process: protein polymerization; striated muscle contraction; regulation of heart rate; negative regulation of potassium ion transport; sequestering of calcium ion; sarcomere organization; detection of calcium ion; transmembrane transport; cardiac muscle contraction

Disease: Ventricular Tachycardia, Catecholaminergic Polymorphic, 1, With Or Without Atrial Dysfunction And/or Dilated Cardiomyopathy; Ventricular Tachycardia, Catecholaminergic Polymorphic, 2