Mouse anti-Human MAPRE2 / EB2 Monoclonal Antibody | anti-MAPRE2 antibody

Mouse Monoclonal [clone 1F3] (IgG2a) to Human MAPRE2 / EB2

Cat. Num. AAA249016

• Gene Names: MAPRE2; EB1; EB2; RP1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G Purified
• Synonyms: MAPRE2 / EB2; Monoclonal Antibody; Mouse Monoclonal [clone 1F3] (IgG2a) to Human MAPRE2 / EB2; Anti-MAPRE2 / EB2 Antibody (clone 1F3) IHC-plus; MAPRE2; APC-binding protein EB2; APC-binding protein EB1; EB1; EB2; End-binding protein 2; Human MAPRE2; anti-MAPRE2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1F3
• Specificity: Human MAPRE2 / EB2
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.81 mg/ml (varies by lot)
• Sequence Length: 327

• Applicable Applications for anti-MAPRE2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:100), IF (1:100), IHC-P (20 ug/ml), WB (1:2000)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
• Immunogen Type: Recombinant protein
• Immunogen: MAPRE2 / EB2 antibody was raised against full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MAPRE2 / EB2 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.)

Immunofluorescence (IF)

(Anti-MAPRE2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPRE2.)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-MAPRE2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAPRE2 antibody, and then analyzed by flow cytometry.)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPRE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPRE2.)

NCBI and Uniprot Product Information

• NCBI GI #: 10346135
• NCBI GeneID: 10982
• NCBI Accession #: NP_055083.1
• NCBI GenBank Nucleotide #: NM_014268.3
• UniProt Accession #: Q15555; Q9UQ33; B2RE21; B3KR39; B4DJV4; B7Z2L3; E9PHR3; F5H1V8; G5E9I6
• Molecular Weight: 30,691 Da
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 2 isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein, RP/EB family, member 2
• NCBI Official Symbol: MAPRE2
• NCBI Official Synonym Symbols: EB1; EB2; RP1
• NCBI Protein Information: microtubule-associated protein RP/EB family member 2; end-binding protein 2; APC-binding protein EB1; APC-binding protein EB2; T-cell activation protein, EB1 family
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 2
• Protein Family: Microtubule-associated protein RP/EB family
• UniProt Gene Name: MAPRE2
• UniProt Synonym Gene Names: RP1; EB2
• UniProt Entry Name: MARE2_HUMAN

NCBI Description

The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

RP1: shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. Its function is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: microtubule cytoskeleton; cytoplasm

Molecular Function: microtubule binding

Biological Process: cell proliferation; mitosis; cell division; signal transduction