Rabbit Topo IIbeta Polyclonal Antibody | anti-TopoIIbeta antibody

Topo IIbeta (A32) Polyclonal Antibody

Cat. Num. AAA3001150

• Gene Names: TOP2B; TOPIIB; top2beta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Topo IIbeta; Polyclonal Antibody; Topo IIbeta (A32) Polyclonal Antibody; TOP2B; DNA topoisomerase 2-beta; DNA topoisomerase II; beta isozyme; anti-TopoIIbeta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Topoisomerase II beta (A32) polyclonal antibody detects endogenous levels of Topoisomerase II beta protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1621

• Applicable Applications for anti-TopoIIbeta antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Topo IIbeta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Topo IIbeta (A32) pAb at 1:500 dilutionLane1:A549 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:AML-12 whole cell lysate (20ug) Lane4:PC12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Topo IIbeta (A32) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Related Product Information for anti-TopoIIbeta antibody

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

NCBI and Uniprot Product Information

• NCBI GI #: 19913408
• NCBI GeneID: 7155
• NCBI Accession #: NP_001059.2
• NCBI GenBank Nucleotide #: NP_001059.2
• UniProt Accession #: Q02880
• Molecular Weight: ~ 183kDa
• NCBI Official Full Name: DNA topoisomerase 2-beta isoform 2
• NCBI Official Synonym Full Names: DNA topoisomerase II beta
• NCBI Official Symbol: TOP2B
• NCBI Official Synonym Symbols: TOPIIB; top2beta
• NCBI Protein Information: DNA topoisomerase 2-beta
• UniProt Protein Name: DNA topoisomerase 2-beta
• UniProt Gene Name: TOP2B
• UniProt Entry Name: TOP2B_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Uniprot Description

TOP2B: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation; Nucleolus; Topoisomerase; EC 5.99.1.3; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p24

Cellular Component: nucleoplasm; heterochromatin; DNA topoisomerase complex (ATP-hydrolyzing); cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; enzyme binding; protein kinase C binding; protein heterodimerization activity; metal ion binding; histone deacetylase binding; chromatin binding; DNA bending activity; ATP binding

Biological Process: DNA unwinding during replication; mitotic recombination; DNA topological change; axonogenesis; forebrain development; sister chromatid segregation; neuron migration; resolution of meiotic joint molecules as recombinants