Rabbit anti-Human Topo IIalpha Polyclonal Antibody | anti-TopoIIalpha antibody

Topo IIalpha (Q14) Polyclonal Antibody

Cat. Num. AAA3003311

• Gene Names: TOP2A; TOP2; TP2A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Topo IIalpha; Polyclonal Antibody; Topo IIalpha (Q14) Polyclonal Antibody; Topoisomerase II alpha 170 kDa; Topo II alpha; Topoisomerase DNA II alpha 170kDa; TopII alpha; TP2A; TOP2A; TOP2; TOP 2A; EC 5.99.1.3; DNA topoisomerase II alpha isozyme; DNA topoisomerase II 170 kD; DNA topoisomerase 2 alpha; DNA Topoisomerase2; anti-TopoIIalpha antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Topo IIalpha (Q14) polyclonal antibody detects endogenous levels of Topo IIalpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1531

• Applicable Applications for anti-TopoIIalpha antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Topo IIalpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Topo IIalpha (Q14) pAb at 1:1000 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (20ug) Lane3:A549 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Topo IIalpha (Q14) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Immunoprecipitation (IP)

(Immunoprecipitation of HepG2 cell lysate usingTopo IIalpha  (Q14) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using Topo IIalpha  (Q14) # MBS3003311. “up-arrow” (supernatant) ; “down-arrow (deposition))

Related Product Information for anti-TopoIIalpha antibody

DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIalpha and Topo IIbeta.

NCBI and Uniprot Product Information

• NCBI GI #: 19913406
• NCBI GeneID: 7153
• NCBI Accession #: NP_001058.2
• NCBI GenBank Nucleotide #: NP_001058.2
• UniProt Accession #: P11388
• Molecular Weight: ~ 174kDa
• NCBI Official Full Name: DNA topoisomerase 2-alpha
• NCBI Official Synonym Full Names: DNA topoisomerase II alpha
• NCBI Official Symbol: TOP2A
• NCBI Official Synonym Symbols: TOP2; TP2A
• NCBI Protein Information: DNA topoisomerase 2-alpha
• UniProt Protein Name: DNA topoisomerase 2-alpha
• UniProt Gene Name: TOP2A
• UniProt Synonym Gene Names: TOP2
• UniProt Entry Name: TOP2A_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Uniprot Description

TOP2A: a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described.

Protein type: Isomerase; EC 5.99.1.3

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleoplasm; centriole; nuclear chromosome; viral integration complex; DNA topoisomerase complex (ATP-hydrolyzing); protein complex; nucleolus; condensed chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; ubiquitin binding; protein homodimerization activity; histone deacetylase binding; magnesium ion binding; drug binding; protein binding; enzyme binding; protein kinase C binding; DNA binding; protein heterodimerization activity; chromatin binding; DNA bending activity; ATP binding

Biological Process: DNA unwinding during replication; mitotic recombination; positive regulation of viral genome replication; DNA topological change; positive regulation of apoptosis; sister chromatid segregation; rhythmic process; apoptotic chromosome condensation; regulation of circadian rhythm; embryonic cleavage; chromosome segregation; positive regulation of retroviral genome replication; DNA ligation; resolution of meiotic joint molecules as recombinants; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; mitotic cell cycle; response to DNA damage stimulus