Western Blot (WB)
Rabbit anti-Human TOP2B Polyclonal Antibody | anti-TOP2B antibody
Anti-TOP2B Antibody
Gene Names
top2b; im:7138121; wu:fc38e02; si:dkey-98n4.1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Protein A purified
Synonyms
TOP2B; Polyclonal Antibody; Anti-TOP2B Antibody; anti-TOP2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
TOP2B antibody detects endogenous levels of TOP2B protein. Not tested with other proteins
Purity/Purification
Protein A purified
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
145
Applicable Applications for anti-TOP2B antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Recommended dilutions:
Peptide ELISA: 1:10,000
WB: 1:100 - 1:500
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: 1:10,000
WB: 1:100 - 1:500
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Synthetic peptide conjugated to KLH
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-TOP2B antibody
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq, Jul 2008]
References
Mirski,S.E., Sparks,K.E., Friedrich,B et,al Topoisomerase II binds importin alpha isoforms and exportin/CRM1 but does not shuttle between the nucleus and cytoplasm inproliferating cells
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,435 Da
NCBI Official Full Name
Top2b protein, partial
NCBI Official Synonym Full Names
topoisomerase (DNA) II beta
NCBI Official Symbol
top2b
NCBI Official Synonym Symbols
im:7138121; wu:fc38e02; si:dkey-98n4.1
NCBI Protein Information
DNA topoisomerase 2-beta
UniProt Protein Name
Top2b protein
Protein Family
UniProt Gene Name
top2b
UniProt Entry Name
A3KNQ9_DANRE
Research Articles on TOP2B
Similar Products
Product Notes
The TOP2B top2b (Catalog #AAA416285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TOP2B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOP2B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Recommended dilutions: Peptide ELISA: 1:10,000 WB: 1:100 - 1:500 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Researchers should empirically determine the suitability of the TOP2B top2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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