Immunohistochemistry - Paraffin (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Mouse anti-Human EIF4E Monoclonal Antibody | anti-EIF4E antibody
Anti-EIF4E Antibody IHC-plus
IHC-P (1:100)
WB (1:100 - 1:500)
For long term storage store at -20 degree C.
Immunohistochemistry - Paraffin (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry - Paraffin (IHC)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Fluorescent image of HeLa cell stained with EIF4E Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EIF4E immunoreactivity is localized to Cytoplasm significantly.)
Immunofluorescence (IF)
(Fluorescent image of HeLa cell stained with EIF4E Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EIF4E immunoreactivity is localized to Cytoplasm significantly.)
Western Blot (WB)
(EIF4E Antibody western blot of HeLa cell line lysates (35 ug/lane). The EIF4E antibody detected the EIF4E protein (arrow).)
Western Blot (WB)
(EIF4E Antibody western blot of HeLa cell line lysates (35 ug/lane). The EIF4E antibody detected the EIF4E protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot Description
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Research Articles on EIF4E
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Product Notes
The EIF4E eif4e (Catalog #AAA249294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-EIF4E Antibody IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4E can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB). IF (1:10 - 1:50) IHC-P (1:100) WB (1:100 - 1:500). Researchers should empirically determine the suitability of the EIF4E eif4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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