Rabbit Toll-like Receptor 3 (TLR3) Polyclonal Antibody | anti-Tlr3 antibody

Toll-like Receptor 3 (TLR3) Polyclonal Antibody

Cat. Num. AAA194448

• Gene Names: Tlr3; Tlr3
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Toll-like Receptor 3 (TLR3); Polyclonal Antibody; Toll-like Receptor 3 (TLR3) Polyclonal Antibody; anti-Tlr3 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Toll-like Receptor 3 (TLR3) [Rabbit polyclonal antibody recognizes human and mouse TLR3]; This antibody recognizes human and mouse TLR3.
• Form/Format: 50 ug purified by antigen affinity chromatography in PBS containing 0.02% sodium azide.
• Sequence Length: 905

• Applicable Applications for anti-Tlr3 antibody: Western Blot, Immunohistochemistry
• Application Notes: Western Blotting: use at 1-2ug/ml. A band of approximately 97 kDa is detected.; Immunohistochemistry: formalin-fixed, paraffin-embedded tissues, use at 5ug/ml.
• Antigen: Synthetic peptide corresponding to 15 amino acids near the N-terminus of human TLR3.
• Preservatives: 0.02% sodium azide
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: Store at 4 degree C. Avoid freezing.

Immunohistochemistry

Related Product Information for anti-Tlr3 antibody

TLR3 is a 904 aa protein with 24 N-terminal leucine-rich repeat motifs (LRRs) and a molecular weight of 97 kDa. TLR3 is most closely related to TLR5, TLR7, and TLR8, each with 26% overall sequence identity. In vivo, two different sized transcripts for TLR3 are observed suggesting that the mRNA is alternatively spliced to generate two different forms of the protein. TLR3 mRNA is expressed at highest levels in placenta and pancreas. There are conflicting reports regarding the expression of TLR3 in particular leukocyte populations. Some suggest that TLR3 is only expressed by dendritic cells (DCs), while others find that TLR3 is expressed by T or NK cells. While it is generally assumed that TLRs are expressed on the cell surface, it now appears that some, such as TLR3, may be localized intracellularly, perhaps in the lysosomal compartment.

Product Categories/Family for anti-Tlr3 antibody

Neuroscience and Signal Transduction Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 38454316
• NCBI GeneID: 364594
• NCBI Accession #: NP_942086.1
• NCBI GenBank Nucleotide #: NM_198791.1
• UniProt Accession #: Q7TNI8; Q80Z88
• Molecular Weight: 103,175 Da
• NCBI Official Full Name: toll-like receptor 3
• NCBI Official Synonym Full Names: toll-like receptor 3
• NCBI Official Symbol: Tlr3
• NCBI Official Synonym Symbols: Tlr3
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr3
• UniProt Entry Name: Q7TNI8_RAT

NCBI Description

human homolog induces activation of NF-kappaB; mouse homolog plays a role in the production of inflammatory cytokines [RGD]

Uniprot Description

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: cell surface; lysosomal membrane; cytoplasm; integral to membrane; intracellular

Molecular Function: identical protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity

Biological Process: positive regulation of interleukin-12 production; positive regulation of apoptosis; microglial cell activation; positive regulation of JNK cascade; defense response; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; defense response to virus; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; response to virus; male gonad development; response to dsRNA; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; positive regulation of chemokine biosynthetic process; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; I-kappaB phosphorylation; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response