Western Blot (WB)
(Western blot analysis of tNOX expression in SW480 (A), Jurkat (B), mouse kidney (C), mouse ovary (D), rat liver (E) whole cell lysates.)
Rabbit tNOX Polyclonal Antibody | anti-ENOX2 antibody
Anti-tNOX Antibody
Western Blot (WB)
(Western blot analysis of tNOX expression in SW480 (A), Jurkat (B), mouse kidney (C), mouse ovary (D), rat liver (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of tNOX expression in SW480 (A), Jurkat (B), mouse kidney (C), mouse ovary (D), rat liver (E) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of tNOX staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of tNOX staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a tumor-specific member of the ECTO-NOX family of genes that encode cell surface NADH oxidases. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The protein also displays prion-like properties. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
ENOX2: May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide- thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock. Belongs to the ENOX family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.-.-.-; Cytoskeletal; Cell surface
Chromosomal Location of Human Ortholog: Xq25
Cellular Component: cytosol; external side of plasma membrane; extracellular space
Molecular Function: nucleic acid binding; nucleotide binding; protein binding; protein disulfide oxidoreductase activity
Biological Process: cell growth; regulation of growth; ultradian rhythm
Research Articles on ENOX2
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Product Notes
The ENOX2 enox2 (Catalog #AAA8245578) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-tNOX Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's tNOX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/100. Researchers should empirically determine the suitability of the ENOX2 enox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "tNOX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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