Rabbit anti-Human TNIK Polyclonal Antibody | anti-TNIK antibody

TNIK (S764) (TRAF2 and NCK-interacting Kinase, KIAA0551) (FITC)

Cat. Num. AAA6503238

• Gene Names: TNIK; MRT54
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TNIK; Polyclonal Antibody; TNIK (S764) (TRAF2 and NCK-interacting Kinase; KIAA0551) (FITC); anti-TNIK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TNIK.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TNIK antibody: FLISA, Western Blot (WB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic non-phosphopeptide selected from the region encoding serine at position 764 of human TNIK (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-TNIK-pS764 Pab and TNIK-pS764 Pab (AP7970d)on nitrocellulose membrane. 50ng of Phospho-peptide (BP3276a) or Non Phospho-peptide (BP7970d) per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.)

Product Categories/Family for anti-TNIK antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 55741807
• NCBI GeneID: 23043
• NCBI Accession #: NP_055843
• NCBI GenBank Nucleotide #: NM_015028.3
• UniProt Accession #: Q9UKE5; O60298; Q8WUY7; Q9UKD8; Q9UKD9; Q9UKE0; Q9UKE1; Q9UKE2; Q9UKE3; A7E2A3; A8K4U1; D3DNQ6
• Molecular Weight: 144,251 Da
• NCBI Official Full Name: TRAF2 and NCK-interacting protein kinase isoform 1
• NCBI Official Synonym Full Names: TRAF2 and NCK interacting kinase
• NCBI Official Symbol: TNIK
• NCBI Official Synonym Symbols: MRT54
• NCBI Protein Information: TRAF2 and NCK-interacting protein kinase
• UniProt Protein Name: TRAF2 and NCK-interacting protein kinase
• Protein Family: TRAF2 and NCK-interacting protein kinase
• UniProt Gene Name: TNIK
• UniProt Synonym Gene Names: KIAA0551
• UniProt Entry Name: TNIK_HUMAN

NCBI Description

Wnt signaling plays important roles in carcinogenesis and embryonic development. The protein encoded by this gene is a serine/threonine kinase that functions as an activator of the Wnt signaling pathway. Mutations in this gene are associated with an autosomal recessive form of cognitive disability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2017]

Uniprot Description

TNIK: Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322. Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1. Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: nucleoplasm; recycling endosome; cytoskeleton; apical plasma membrane; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: microvillus biogenesis; Wnt receptor signaling pathway; protein amino acid autophosphorylation; regulation of dendrite morphogenesis; actin cytoskeleton reorganization; cytoskeleton organization and biogenesis; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; activation of JNKK activity