Immunofluorescence (IF)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.)
Chicken Ankyrin 3/Ankyrin G Polyclonal Antibody | anti-ANK3 antibody
Ankyrin 3/Ankyrin G
IHC: 1:500-1,000
WB: 1:5,000-10,000
Immunofluorescence (IF)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.)
Immunofluorescence (IF)
(Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with chicken pAb to ankyrin 3, CH22125, dilution 1:2,000 in green, and costained with mouse mAb to FOX3/NeuN dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The CH22125 antibody stains the axonal initial segments, while the FOX3/NeuN antibody reveals perikarya and nuclei of neurons.)
Western Blot (WB)
(Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.)
Western Blot (WB)
(Western blot analysis of different tissue and cell lysates using chicken pAb to ankyrin 3 dilution 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction.)
NCBI and Uniprot Product Information
NCBI Description
Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact, and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 3 is an immunologically distinct gene product from ankyrins 1 and 2, and was originally found at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
Uniprot Description
ANK3: In skeletal muscle, required for costamere localization of DMD and betaDAG1. Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. Genetic variations in ANK3 are associated with autism spectrum disorders susceptibility. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 10q21
Cellular Component: Golgi apparatus; tight junction; costamere; cell surface; sarcoplasmic reticulum; endoplasmic reticulum; basolateral plasma membrane; lysosome; paranode region of axon; T-tubule; dendrite; Z disc; postsynaptic membrane; plasma membrane; basal plasma membrane; neuromuscular junction; sarcolemma; lateral plasma membrane
Molecular Function: protein binding, bridging; protein binding; spectrin binding; structural constituent of cytoskeleton; cadherin binding; cytoskeletal protein binding
Biological Process: axon guidance; regulation of potassium ion transport; cytoskeletal anchoring; generation of action potential; signal transduction; positive regulation of homotypic cell-cell adhesion; establishment of protein localization; cytokinesis after mitosis; plasma membrane organization and biogenesis; axonogenesis; Golgi to plasma membrane protein transport; positive regulation of membrane potential; neuromuscular junction development
Disease: Mental Retardation, Autosomal Recessive 37
Research Articles on ANK3
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Product Notes
The ANK3 ank3 (Catalog #AAA555975) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Ankyrin 3/Ankyrin G reacts with Human, Mouse, Rat, Primate, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's Ankyrin 3/Ankyrin G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). IF: 1:500-1,000 IHC: 1:500-1,000 WB: 1:5,000-10,000. Researchers should empirically determine the suitability of the ANK3 ank3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ankyrin 3/Ankyrin G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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