Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit | FASL elisa kit

Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit

Cat. Num. AAA451244

• Gene Names: Faslg; Fasl; CD95-L; Tnfsf6; Tnlg1a; Apt1Lg1
• Reactivity: Rat
• Synonyms: Factor Related Apoptosis Ligand (FASL); Rat Factor Related Apoptosis Ligand (FASL) ELISA Kit; CD178; CD95L; CD95-L; FASLG; APT1LG1; APT1-LG1; TNFSF6; Fas Antigen Ligand; TNF Superfamily Member 6; Tumor Necrosis Factor(ligand)Superfamily; Member 6; FASL elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of FASL. No significant cross-reactivity or interference between FASL and analogues was observed.
• Sequence Length: 278

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.076ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FASL were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FASL were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FASL elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FASL in rat serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FASL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FASL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FASL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FASL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978525
• NCBI GeneID: 25385
• NCBI Accession #: NP_037040.1
• NCBI GenBank Nucleotide #: NM_012908.1
• UniProt Accession #: P36940
• Molecular Weight: 31,140 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 6
• NCBI Official Synonym Full Names: Fas ligand
• NCBI Official Symbol: Faslg
• NCBI Official Synonym Symbols: Fasl; CD95-L; Tnfsf6; Tnlg1a; Apt1Lg1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• UniProt Gene Name: Faslg
• UniProt Synonym Gene Names: Apt1Lg1; Cd95l; Fasl; Tnfsf6; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA

NCBI Description

ligand that binds the Fas receptor; plays a role in induction of apoptosis [RGD, Feb 2006]

Uniprot Description

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects ().