17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12) Recombinant Protein | HSD17b12 recombinant protein

Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12)

Cat. Num. AAA2011648

• Gene Names: Hsd17b12; Kik1; KIK-I; AI172963; 2610510O05Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12); Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12); HSD17b12 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-MECAPPAAGF LYWVGASTIA YLALRASYSL FRAFQVWCVG NEALVGPRLG EWAVVTGGTD GIGKAYAEEL AKRGMKIVLI SRSQDKLNQV SNNIKEKFNV ETRTIAVDFS LDDIYDKIKT GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL DNTIKKLINI NVLSVCKVTR LVLPGMVERS KGVILNISSA SGMLPVPLLT IYSATKAFVD FFSQCLHEEY KSKGIFVQSV MPYLVATKLA KIQKPTLDKP SAETFVKSAI KTVGLQTRTT GYVIHSLMGS INSIMPRWMY FKIIMGFSKS LRNRYLKKRK KN
• Sequence Length: 312

• Applicable Applications for HSD17b12 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Asn312 (Accession # O70503) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9789991
• NCBI GeneID: 56348
• NCBI Accession #: NP_062631.1
• NCBI GenBank Nucleotide #: NM_019657.4
• UniProt Accession #: O70503; Q3TID1; Q3U7V9; Q542N3; Q8CI39
• Molecular Weight: 61.8kDa
• NCBI Official Full Name: estradiol 17-beta-dehydrogenase 12
• NCBI Official Synonym Full Names: hydroxysteroid (17-beta) dehydrogenase 12
• NCBI Official Symbol: Hsd17b12
• NCBI Official Synonym Symbols: Kik1; KIK-I; AI172963; 2610510O05Rik
• NCBI Protein Information: estradiol 17-beta-dehydrogenase 12; KAR; keratonectin; keratoadhesin; 17-beta-HSD 12; steroid dehydrogenase; 3-ketoacyl-CoA reductase; 17-beta-hydroxysteroid dehydrogenase 12
• UniProt Protein Name: Estradiol 17-beta-dehydrogenase 12
• Protein Family: Very-long-chain 3-oxoacyl-CoA reductase
• UniProt Gene Name: Hsd17b12
• UniProt Synonym Gene Names: Kik1; 17-beta-HSD 12; KAR
• UniProt Entry Name: DHB12_MOUSE

Uniprot Description

HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 1.1.1.62; Membrane protein, integral; Oxidoreductase; Lipid Metabolism - androgen and estrogen; Membrane protein, multi-pass

Cellular Component: proteinaceous extracellular matrix; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: heparin binding; estradiol 17-beta-dehydrogenase activity; cis-chlorobenzene dihydrodiol dehydrogenase activity; cinnamate reductase activity; oxidoreductase activity; cis-2,3-dihydrodiol DDT dehydrogenase activity; collagen binding; 2,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase activity; trans-1,2-dihydrodiolphenanthrene dehydrogenase activity; fibronectin binding; trans-9R,10R-dihydrodiolphenanthrene dehydrogenase activity; enoyl-[acyl-carrier-protein] reductase activity; 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase activity

Biological Process: extracellular matrix organization and biogenesis; metabolic process; lipid metabolic process; steroid biosynthetic process