Tnfsf11 antibody pair

Tnfsf11 Antibody Pair

Cat. Num. AAA7041921

• Gene Names: Tnfsf11; RANKL
• Reactivity: Human, Mouse, Guinea Pig, Pig, Dog
• Applications: ELISA
• Synonyms: Tnfsf11; Polyclonal Antibody Pair; Tnfsf11 Antibody Pair; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD_antigen=CD254; Tnfsf11 antibody pair

• Host: Capture: Rabbit; Detection: Rabbit
• Reactivity: Human, Mouse, Guinea Pig, Pig, Dog
• Clonality: Polyclonal
• Isotype: Capture: IgG; Detection: IgG
• Form/Format: Liquid
• Concentration: The concentrations and volumes of the antibodies may vary and be lot specific. Details will be on the vial labels. (varies by lot)
• Sequence Length: 318

• Applicable Applications for Tnfsf11 antibody pair: Sandwich ELISA (EIA)
• Application Notes: We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.2ug/ml.
• Immunogen Species: Mouse
• Conjugation: Capture: Non-conjugated; Detection: Biotin conjugated
• Recommended Standard: COLO 320 cell; HEK-293 cell; (Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.
• Storage Buffer: Capture: 50% Glycerol, 0.01M PBS, PH 7.4; Detection: 50% Glycerol, 0.01M PBS, PH 7.4
• Other Note: Immunogen information is company proprietary information. Antibodies are sold as a pair and not available separately.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze/thaw cycles. When stored properly, products should be stable for at least 5 months.

NCBI and Uniprot Product Information

• NCBI GI #: 16924012
• NCBI GeneID: 117516
• NCBI Accession #: NP_476490.1
• NCBI GenBank Nucleotide #: NM_057149.1
• Molecular Weight: 35,370 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: RANKL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_RAT

NCBI Description

induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]

Uniprot Description

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.