Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) RTU ELISA Kit | RANkL rtu elisa kit

Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit

Cat. Num. AAA4502172

• Gene Names: Tnfsf11; RANKL
• Reactivity: Rat
• Synonyms: Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL); Rat Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) ELISA Kit; CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine; RANkL rtu elisa kit

• Reactivity: Rat
• Sequence Length: 318

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.3pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RANkL rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RANkL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RANkL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RANkL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RANkL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 16924012
• NCBI GeneID: 117516
• NCBI Accession #: NP_476490.1
• NCBI GenBank Nucleotide #: NM_057149.1
• UniProt Accession #: Q9ESE2; Q91ZI9
• Molecular Weight: 35,370 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: TNF superfamily member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: RANKL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE

NCBI Description

induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]

Uniprot Description

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.