Rabbit anti-Human TNFRSF14 Polyclonal Antibody | anti-TNFRSF14 antibody

TNFRSF14, ID (TNFRSF14, HVEA, HVEM, Tumor necrosis factor receptor superfamily member 14, Herpes virus entry mediator A, Tumor necrosis factor receptor-like 2, CD270) (Biotin)

Cat. Num. AAA6356946

• Gene Names: TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TNFRSF14; Polyclonal Antibody; ID (TNFRSF14; HVEA; HVEM; Tumor necrosis factor receptor superfamily member 14; Herpes virus entry mediator A; Tumor necrosis factor receptor-like 2; CD270) (Biotin); CD270; anti-TNFRSF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TNFRSF14.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TNFRSF14 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 210-236 in the Central region of human TNFRSF14, UniProt Accession #Q92956.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HL-60 cell line lysate using MBS6000750 pre-incubated without (lane 1) and with (lane 2) blocking peptide. TNFRSF14 (arrow) was detected usingMBS6000750.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ormalin-fixed paraffin-embedded human lung carcinoma usingMBS6000750, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6000750 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram), usingMBS6000750. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TNFRSF14 antibody

Antibodies; Receptors

References

1. Dubois, P.C., et.al., Nat. Genet. 42(4):295-302 (2010). 2. Davila, S., et.al., Genes Immun. (2010). In press.

NCBI and Uniprot Product Information

• NCBI GI #: 13878821
• NCBI GeneID: 8764
• UniProt Accession #: Q92956; Q6IB95; Q8N634; Q8WXR1; Q96J31; Q9UM65; B3KW30; B9DI89
• Molecular Weight: 283
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 14
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 14
• NCBI Official Symbol: TNFRSF14
• NCBI Official Synonym Symbols: TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 14; CD40-like protein; herpes virus entry mediator A; tumor necrosis factor receptor-like gene2; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 14
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF14
• UniProt Synonym Gene Names: HVEA; HVEM; Herpesvirus entry mediator A; HveA; TR2
• UniProt Entry Name: TNR14_HUMAN

NCBI Description

This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFRSF14: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.

Protein type: Receptor, misc.; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: viral receptor activity; protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; entry of virus into host cell; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; T cell costimulation; positive regulation of cytokine secretion during immune response; negative regulation of alpha-beta T cell proliferation; immune response; defense response to Gram-negative bacterium