Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM70 (TOMM70A) Standard Curve.)
Human Mitochondrial Import Receptor Subunit TOM70 (TOMM70A) ELISA Kit | TOMM70A elisa kit
Human Mitochondrial Import Receptor Subunit TOM70 (TOMM70A) ELISA Kit
Gene Names
TOMM70; Tom70; TOMM70A
Reactivity
Human
Synonyms
Mitochondrial Import Receptor Subunit TOM70 (TOMM70A); Human Mitochondrial Import Receptor Subunit TOM70 (TOMM70A) ELISA Kit; TOMM70A; FLJ90470; translocase of outer mitochondrial membrane 70 homolog A; TOMM70A elisa kit
Reactivity
Human
Specificity
Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM70A. No significant cross-reactivity or interference between Human TOMM70A and analogues was observed.
Sequence Length
608
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM70 (TOMM70A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Mitochondrial import receptor subunit TOM70 (TOMM70A) Standard Curve.)
Related Product Information for TOMM70A elisa kit
Description: This Human Mitochondrial import receptor subunit TOM70 (TOMM70A) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM70A in samples. An antibody specific for TOMM70A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOMM70A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM70A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM70A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria.TOMM70A is 29% identical to the mitochondrial precursor protein import receptor of Neurospora crassa. RT-PCR analysis detected ubiquitous expression of KIAA0719, with relatively weak expression in pancreas, testis, and spleen.the cytosolic chaperones HSP90 and HSP70 dock onto a specialized tetratricopeptide domain in the import receptor TOMM70 at the outer mitochondrial membrane. This interaction served to deliver a set of preproteins to the receptor for subsequent membrane translocation dependent on the HSP90 ATPase. Disruption of the chaperone/TOMM70 recognition inhibited the import of these preproteins into mitochondria.
Background: The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria.TOMM70A is 29% identical to the mitochondrial precursor protein import receptor of Neurospora crassa. RT-PCR analysis detected ubiquitous expression of KIAA0719, with relatively weak expression in pancreas, testis, and spleen.the cytosolic chaperones HSP90 and HSP70 dock onto a specialized tetratricopeptide domain in the import receptor TOMM70 at the outer mitochondrial membrane. This interaction served to deliver a set of preproteins to the receptor for subsequent membrane translocation dependent on the HSP90 ATPase. Disruption of the chaperone/TOMM70 recognition inhibited the import of these preproteins into mitochondria.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Mitochondrial import receptor subunit TOM70
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 70
NCBI Official Symbol
TOMM70
NCBI Official Synonym Symbols
Tom70; TOMM70A
NCBI Protein Information
mitochondrial import receptor subunit TOM70
UniProt Protein Name
Mitochondrial import receptor subunit TOM70
UniProt Gene Name
TOMM70A
UniProt Synonym Gene Names
KIAA0719; TOM70
UniProt Entry Name
TOM70_HUMAN
NCBI Description
This gene encodes an import receptor of the outer mitochondrial membrane that is part of the translocase of the outer membrane complex. This protein is involved in the import of mitochondrial precursor proteins. [provided by RefSeq, Oct 2011]
Uniprot Description
TOMM70A: Receptor that accelerates the import of all mitochondrial precursor proteins. Belongs to the Tom70 family.
Protein type: Receptor, protein translocating; Mitochondrial; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q12.2
Cellular Component: membrane; mitochondrial outer membrane translocase complex; mitochondrion; integral to membrane
Molecular Function: protein binding; protein transmembrane transporter activity
Biological Process: cellular protein metabolic process; protein targeting to mitochondrion
Research Articles on TOMM70A
Similar Products
Product Notes
The Human TOMM70A tomm70a (Catalog #AAA9713827) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713827 ELISA Kit recognizes Human TOMM70A. It is sometimes possible for the material contained within the vial of "Mitochondrial Import Receptor Subunit TOM70 (TOMM70A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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