Rabbit TNFAIP3 Polyclonal Antibody | anti-Tnfaip3 antibody

TNFAIP3 Antibody

Cat. Num. AAA151182

• Gene Names: Tnfaip3; A20; Tnfip3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TNFAIP3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TNFAIP3; Polyclonal Antibody; TNFAIP3 Antibody; A20; Tnfip3; Tumor necrosis factor alpha-induced protein 3; Putative DNA-binding protein A20; TNF alpha-induced protein 3; tumor necrosis factor; alpha-induced protein 3; anti-Tnfaip3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TNFAIP3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 775

• Applicable Applications for anti-Tnfaip3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TNFAIP3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TNFAIP3.
• Buffer: TNFAIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TNFAIP3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TNFAIP3 in Jurkat cell lysate with TNFAIP3 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 antibody at 20 μg/mL.)

Related Product Information for anti-Tnfaip3 antibody

TNFAIP3 Antibody: TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-α, inhibiting NF-κB-dependent gene expression, and protecting cells from TNF-α-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 2498097
• NCBI GeneID: 21929
• UniProt Accession #: Q60769; Q3U968
• Molecular Weight: 87,654 Da
• NCBI Official Full Name: Tumor necrosis factor alpha-induced protein 3
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 3
• NCBI Official Symbol: Tnfaip3
• NCBI Official Synonym Symbols: A20; Tnfip3
• NCBI Protein Information: tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; putative DNA-binding protein A20; tumor necrosis factor induced protein 3
• UniProt Protein Name: Tumor necrosis factor alpha-induced protein 3
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: Tnfaip3
• UniProt Synonym Gene Names: Tnfip3; TNF alpha-induced protein 3
• UniProt Entry Name: TNAP3_MOUSE

Uniprot Description

TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.

Protein type: Protease; Apoptosis; Ubiquitin conjugating system; EC 3.4.19.12

Cellular Component: centrosome; lysosome; cytoplasm; nucleus

Molecular Function: ubiquitin binding; protease binding; protein self-association; zinc ion binding; hydrolase activity; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ubiquitin-specific protease activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; peptidase activity; protein binding; DNA binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; kinase binding; catalytic activity; cysteine-type peptidase activity; ligase activity

Biological Process: negative regulation of autophagy; negative regulation of toll-like receptor 5 signaling pathway; metabolic process; apoptosis; negative regulation of smooth muscle cell proliferation; regulation of immunoglobulin production; proteolysis; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of interleukin-2 production; negative regulation of innate immune response; response to molecule of bacterial origin; negative regulation of interleukin-6 production; response to wounding; inflammatory response; negative regulation of chronic inflammatory response; protein deubiquitination; negative regulation of heterotypic cell-cell adhesion; B-1 B cell homeostasis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of B cell activation; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; regulation of germinal center formation; marginal zone B cell differentiation; regulation of innate immune response; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein ubiquitination; negative regulation of granuloma formation