Rabbit anti-Human, Mouse ABIN3 Polyclonal Antibody | anti-TNIP3 antibody

ABIN3 Antibody

Cat. Num. AAA151164

• Gene Names: TNIP3; LIND; ABIN-3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: ABIN3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ABIN3; Polyclonal Antibody; ABIN3 Antibody; LIND; ABIN-3; TNFAIP3-interacting protein 3; A20-binding inhibitor of NF-kappa-B activation 3; TNFAIP3 interacting protein 3; anti-TNIP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ABIN3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 325

• Applicable Applications for anti-TNIP3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ABIN3 antibody can be used for detection of ABIN3 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.; Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: ABIN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ABIN3.; The immunogen is located within the last amino acids of ABIN3
• Buffer: ABIN3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ABIN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 μg/mL.)

Related Product Information for anti-TNIP3 antibody

ABIN3 Antibody: The nuclear factor NF-κB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-κB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-κB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-κB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-κB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-κB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 192807305
• NCBI GeneID: 79931
• NCBI Accession #: NP_079149
• NCBI GenBank Nucleotide #: NM_024873.5
• UniProt Accession #: Q96KP6; Q96PQ3; Q9H780; A1A574; A8K2Z4; B4DVF5; B4E023
• NCBI Official Full Name: TNFAIP3-interacting protein 3 isoform 1
• NCBI Official Synonym Full Names: TNFAIP3 interacting protein 3
• NCBI Official Symbol: TNIP3
• NCBI Official Synonym Symbols: LIND; ABIN-3
• NCBI Protein Information: TNFAIP3-interacting protein 3; TNIP3 beta; ABIN-3 beta; Listeria induced; listeria-induced gene protein; TNFAIP3-interacting protein 3 beta; A20-binding inhibitor of NF-kappa-B activation 3
• UniProt Protein Name: TNFAIP3-interacting protein 3
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: TNIP3
• UniProt Synonym Gene Names: ABIN-3
• UniProt Entry Name: TNIP3_HUMAN

Uniprot Description

TNIP3: Binds to zinc finger protein TNFAIP3 and inhibits NF- kappa-B activation induced by tumor necrosis factor, Toll-like receptor 4 (TLR4), interleukin-1 and 12-O-tetradecanoylphorbol-13- acetate. Overexpression inhibits NF-kappa-B-dependent gene expression in response to lipopolysaccharide at a level downstream of TRAF6 and upstream of IKBKB. NF-kappa-B inhibition is independent of TNFAIP3 binding.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 4q27

Molecular Function: polyubiquitin binding

Biological Process: MyD88-independent toll-like receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; toll-like receptor 4 signaling pathway