Immunofluorescence (IF)
(MBS9600264 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse TNF14 Polyclonal Antibody | anti-TNF14 antibody
TNF14 Antibody
Gene Names
TNFSF14; LTg; CD258; HVEML; LIGHT
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TNF14; Polyclonal Antibody; TNF14 Antibody; CD 258; CD258; CD258 antigen; Delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator A; Herpesvirus entry mediator ligand; herpesvirus entry mediator-ligand; HVEM L; HVEM-L; HVEML; Ligand for herpesvirus entry mediator; LIGHT; LTg; soluble form; TNF14_HUMAN; TNFSF 14; Tnfsf14; TNFSF14 protein; TR 2; TR2; Tumor necrosis factor (ligand) superfamily; member 14; Tumor necrosis factor ligand superfamily member 14; Tumor necrosis factor ligand superfamily; Tumor necrosis factor receptor like 2; tumor necrosis factor receptor-like 2; Tumor necrosis factor superfamily member 14; Tumor necrosis factor superfamily member LIGHT; anti-TNF14 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
TNF14 antibody detects endogenous levels of TNF14
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
240
Applicable Applications for anti-TNF14 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human TNF14
Subcellular Location
Cytoplasm; Secreted and Cell Membrane.
Tissue Specificity
Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
Predicted Cross Reactivity
Dog
Similarity
Dog (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9600264 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9600264 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-TNF14 antibody
Description: Receptor for BTLA. Receptor for TNFSF14/LIGHT and homotrimeric TNFSF1/lymphotoxin-alpha. Involved in lymphocyte activation. Plays an important role in HSV pathogenesis because it enhanced the entry of several wild-type HSV strains of both serotypes into CHO cells, and mediated HSV entry into activated human T-cells.
Function: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
Subunit Structure: Homotrimer.
Post-translational Modifications: N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
Similarity: Belongs to the tumor necrosis factor family.
Function: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
Subunit Structure: Homotrimer.
Post-translational Modifications: N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
Similarity: Belongs to the tumor necrosis factor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 26 kDa
Predicted: 27 kDa
Predicted: 27 kDa
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 14 isoform 1
NCBI Official Synonym Full Names
TNF superfamily member 14
NCBI Official Symbol
TNFSF14
NCBI Official Synonym Symbols
LTg; CD258; HVEML; LIGHT
NCBI Protein Information
tumor necrosis factor ligand superfamily member 14
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 14
UniProt Gene Name
TNFSF14
UniProt Synonym Gene Names
HVEML; LIGHT; HVEM-L; Herpesvirus entry mediator ligand
NCBI Description
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
Research Articles on TNF14
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Product Notes
The TNF14 tnfsf14 (Catalog #AAA9600264) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNF14 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TNF14 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TNF14 tnfsf14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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