Mouse gamma-Tubulin Antibody | anti-TUBG1 antibody

Anti-gamma-Tubulin Purified

Cat. Num. AAA1801632

• Gene Names: TUBG1; TUBG; GCP-1; CDCBM4; TUBGCP1
• Reactivity: Protozoa, Plants, Animals
• Applications: Western Blot, Immunocytochemistry
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: gamma-Tubulin; Anti-gamma-Tubulin Purified; tubulin gamma 1; TUBG; anti-TUBG1 antibody

• Host: Mouse
• Reactivity: Protozoa, Plants, Animals
• Isotype: IgG1
• Clone Number: TU-32
• Specificity: The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-TUBG1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen: human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis of gamma-tubulin in porcine brain lysate by antibody TU-32.)

Related Product Information for anti-TUBG1 antibody

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. Humans possess two gamma-tubulin genes. Gamma-tubulin 1 represents a ubiquitous isotype, whereas gamma-tubulin 2 is found predominantly in the brain, where it may be endowed with divergent functions beyond microtubule nucleation.

NCBI and Uniprot Product Information

• NCBI GI #: 31543831
• NCBI GeneID: 7283
• NCBI Accession #: NP_001061.2
• NCBI GenBank Nucleotide #: NP_001061.2
• UniProt Accession #: P23258
• NCBI Official Full Name: tubulin gamma-1 chain
• NCBI Official Synonym Full Names: tubulin gamma 1
• NCBI Official Symbol: TUBG1
• NCBI Official Synonym Symbols: TUBG; GCP-1; CDCBM4; TUBGCP1
• NCBI Protein Information: tubulin gamma-1 chain
• UniProt Protein Name: Tubulin gamma-1 chain
• Protein Family: Gamma-tubulin complex
• UniProt Gene Name: TUBG1
• UniProt Synonym Gene Names: TUBG; GCP-1
• UniProt Entry Name: TBG1_HUMAN

NCBI Description

This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]

Uniprot Description

TUBG1: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Interacts with GCP2 and GCP3. Interacts with B9D2. Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO/C1orf88. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: centriole; centrosome; recycling endosome; pericentriolar material; condensed nuclear chromosome; cytoplasmic microtubule; nonmotile primary cilium; apical part of cell; cytoplasm; leading edge; polar microtubule; cytosol; gamma-tubulin complex

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: metabolic process; organelle organization and biogenesis; mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; G2/M transition of mitotic cell cycle; cytoplasmic microtubule organization and biogenesis; meiotic spindle organization and biogenesis

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 4