Rabbit TMEM16A Polyclonal Antibody | anti-ANO1 antibody
Anti-TMEM16A antibody
Gene Names
ANO1; DOG1; TAOS2; ORAOV2; TMEM16A
Reactivity
RatPredicted: Human, Mouse
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
TMEM16A; Polyclonal Antibody; Anti-TMEM16A antibody; anti-ANO1 antibody
Host
Rabbit
Reactivity
Rat
Predicted: Human, Mouse
Predicted: Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; each vial contains 4mg Trehalose , 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
Sequence Length
986
Applicable Applications for anti-ANO1 antibody
Western Blot (WB)
Application Notes
Western Blot
Concentration: 0.1-0.5 ug/mL
Tested Species: Rat
Predicted Species: Human, Mouse
Antigen Retrieval: -
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration: 0.1-0.5 ug/mL
Tested Species: Rat
Predicted Species: Human, Mouse
Antigen Retrieval: -
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ANO1 (927-943aa KVLMVELFMREEQDKQQ), different from the related mouse and rat sequences by one amino acid.
Note
Antibody can be supported chemiluminescence kit MBS176460 in Western Blot
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for 6 months.
Avoid repeated freezing and thawing.
It can also be aliquotted and stored frozen at -20°C for 6 months.
Avoid repeated freezing and thawing.
Related Product Information for anti-ANO1 antibody
Anoctamin-1(ANO1), also known as oral cancer overexpressed 2(ORAOV2) or tumor-amplified and overexpressed sequence 2(TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Anoctamin-1
NCBI Official Synonym Full Names
anoctamin 1, calcium activated chloride channel
NCBI Official Symbol
ANO1
NCBI Official Synonym Symbols
DOG1; TAOS2; ORAOV2; TMEM16A
NCBI Protein Information
anoctamin-1; Ca2+-activated Cl- channel; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains)
UniProt Protein Name
Anoctamin-1
Protein Family
UniProt Gene Name
ANO1
UniProt Synonym Gene Names
DOG1; ORAOV2; TAOS2; TMEM16A
UniProt Entry Name
ANO1_HUMAN
Uniprot Description
ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Transporter, ion channel; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13.3
Cellular Component: apical plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity
Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport
Research Articles on ANO1
Similar Products
Product Notes
The ANO1 ano1 (Catalog #AAA176756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMEM16A antibody reacts with Rat Predicted: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM16A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5 ug/mL Tested Species: Rat Predicted Species: Human, Mouse Antigen Retrieval: - Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Researchers should empirically determine the suitability of the ANO1 ano1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM16A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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