Typical Testing Data/Standard Curve
Rat Anoctamin-1 (ANO1) ELISA Kit | ANO1 elisa kit
Rat Anoctamin-1 (ANO1) ELISA Kit
Gene Names
ANO1; DOG1; TAOS2; ORAOV2; TMEM16A
Reactivity
Rat
Synonyms
Anoctamin-1 (ANO1); Rat Anoctamin-1 (ANO1) ELISA Kit; ANO1 elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for ANO1 elisa kit
Intended Uses: This ANO1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ANO1. This ELISA kit for research use only!
Principle of the Assay||ANO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANO1 antibody and an ANO1-HRP conjugate. The assay sample and buffer are incubated together with ANO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO1 concentration since ANO1 from samples and ANO1-HRP conjugate compete for the anti-ANO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO1 from the sample, fewer sites are left to bind ANO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||ANO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANO1 antibody and an ANO1-HRP conjugate. The assay sample and buffer are incubated together with ANO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO1 concentration since ANO1 from samples and ANO1-HRP conjugate compete for the anti-ANO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO1 from the sample, fewer sites are left to bind ANO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ANO1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,369 Da
NCBI Official Full Name
anoctamin-1
NCBI Official Synonym Full Names
anoctamin 1, calcium activated chloride channel
NCBI Official Symbol
ANO1
NCBI Official Synonym Symbols
DOG1; TAOS2; ORAOV2; TMEM16A
NCBI Protein Information
anoctamin-1; Ca2+-activated Cl- channel; oral cancer overexpressed 2; tumor-amplified and overexpressed sequence 2; discovered on gastrointestinal stromal tumors protein 1; transmembrane protein 16A (eight membrane-spanning domains)
UniProt Protein Name
Anoctamin-1
Protein Family
UniProt Gene Name
ANO1
UniProt Synonym Gene Names
DOG1; ORAOV2; TAOS2; TMEM16A
UniProt Entry Name
ANO1_HUMAN
Uniprot Description
ANO1: Acts as a calcium-activated chloride channel. Required for normal tracheal development. Belongs to the anoctamin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13.3
Cellular Component: cytoplasm; apical plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; protein homodimerization activity; intracellular calcium activated chloride channel activity; protein heterodimerization activity; calcium activated cation channel activity
Biological Process: regulation of membrane potential; multicellular organismal development; chloride transport; transmembrane transport; cation transport
Research Articles on ANO1
Similar Products
Product Notes
The Rat ANO1 ano1 (Catalog #AAA7206898) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7206898 ELISA Kit recognizes Rat ANO1. It is sometimes possible for the material contained within the vial of "Anoctamin-1 (ANO1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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