Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TLE1 antibody.)
Rabbit TLE1 Polyclonal Antibody | anti-TLE1 antibody
TLE1 (Groucho 1, ESG, ESG1, GRG1) (PE)
WB: 1:500-1:2000
IHC: 1:50-1:200
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TLE1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TLE1 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using TLE1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U20S cell using TLE1 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. Ref.13
Subunit structure: Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif). Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16
Subcellular location: Nucleus. Note: Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components. Ref.15
Tissue specificity: In all tissues examined, mostly in brain, liver and muscle.
Post-translational modification: Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ref.15Ubiquitinated by XIAP/BIRC4. Ref.21
Sequence similarities: Belongs to the WD repeat Groucho/TLE family.Contains 6 WD repeats.
Research Articles on TLE1
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Product Notes
The TLE1 tle1 (Catalog #AAA6440751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TLE1 (Groucho 1, ESG, ESG1, GRG1) (PE) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TLE1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC). IF: 1:50-1:200 WB: 1:500-1:2000 IHC: 1:50-1:200 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TLE1 tle1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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