Mouse anti-Human PDLIM7 Polyclonal Antibody | anti-PDLIM7 antibody

PDLIM7 (PDZ and LIM Domain Protein 7, LIM Mineralization Protein, LMP, Protein Enigma, ENIGMA)

Cat. Num. AAA6000568

• Gene Names: PDLIM7; LMP1; LMP3
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PDLIM7; Polyclonal Antibody; PDLIM7 (PDZ and LIM Domain Protein 7; LIM Mineralization Protein; LMP; Protein Enigma; ENIGMA); Anti -PDLIM7 (PDZ and LIM Domain Protein 7; anti-PDLIM7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PDLIM7.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MDSFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVIRGRYLVALGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGVPYCERDYEKMFGTKCHGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDRPLCKSHAFSHV

• Applicable Applications for anti-PDLIM7 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human PDLIM7, aa1-457 (NP_005442.2)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 polyclonal antibody. Lane1: PDLIM7 transfected lysate (50.27kD). Lane2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to PDLIM7 on HeLa cell. [antibody concentration 10ug/ml])

Related Product Information for anti-PDLIM7 antibody

PDLIM7 (PDZ and LIM domain 7) is a representative of a family of proteins composed of conserved PDZ and LIM domains. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. It is likely to act as a scaffold for the formation of multiprotein complexes. It may play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. PDLIM7 is involved in the fundamental mechanisms of bone formation and is involved in the BMP signaling pathway. There are six isoforms.

Product Categories/Family for anti-PDLIM7 antibody

Antibodies; Abs to PDZ Domain

NCBI and Uniprot Product Information

• NCBI GI #: 54038515
• NCBI GeneID: 9260
• UniProt Accession #: Q9NR12; Q14250; Q5XG82; Q6NVZ5; Q96C91; Q9BXB8; Q9BXB9
• Molecular Weight: 49,845 Da
• NCBI Official Full Name: PDLIM7 protein
• NCBI Official Synonym Full Names: PDZ and LIM domain 7 (enigma)
• NCBI Official Symbol: PDLIM7
• NCBI Official Synonym Symbols: LMP1; LMP3
• NCBI Protein Information: PDZ and LIM domain protein 7; LMP; 1110003B01Rik; protein enigma; Lim mineralization protein 3
• UniProt Protein Name: PDZ and LIM domain protein 7
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM7
• UniProt Synonym Gene Names: ENIGMA; LMP
• UniProt Entry Name: PDLI7_HUMAN

NCBI Description

The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

PDLIM7: May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation e.g (embryonic flat bones mandible and cranium), and endochondral bone formation e.g (embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: ruffle; focal adhesion; cytoplasm; stress fiber; nucleus; actin cytoskeleton

Molecular Function: protein binding; zinc ion binding

Biological Process: positive regulation of osteoblast differentiation; receptor-mediated endocytosis; ossification; multicellular organismal development; actin cytoskeleton organization and biogenesis; cell differentiation